PAGE gel staining and troubleshooting

This protocol and troubleshooting guide gives some troubleshooting on silver staining of polyacrylamide gel. (Frank lab, Oklahoma Medical Research Foundation)

1. Gray or brown precipitate appearing as smudges or swirling on gel surface. Bands may be faint or absent.
2. Dark uniform background, usually yellow.
3. Mottled background, usually brown or green, with poor sensitivity.
4. Slow or no development.

Last update 06-Jun-2007, Rating n/a of 2 votes.