Rsa digestion.

I am genotyping the mice for defective leptin receptor allele (db). According to the protocol I have to run Rsa digestion on the PCR products in order to digest 135 bp fragments into 108 and 25 bp pieces. It seems to work for heterozygotes (I get two bands), but ... ...

Last update 18-Nov-2000, Rating Good of 0 votes.