Cloning DNA for sequencing

Protocol for cloning DNA for sequencing. (University of Southampton, School of Biological Sciences)

PURIFYING DNA FROM AN AGAR GEL
PHOSPHORYLATING PCR PRODUCTS
LIGATION
PREPARATION OF THE LB-AGAR AND SOC
TRANSFORMATION
PICKING POSITIVE COLONIES
SNAP FREEZING
ALKALINE LYSIS
TEST FOR INSERTION

Last update 12-May-2005, Rating n/a of 0 votes.