Technique / Molecular Biology / Cloning library construction and screening
Typical Cloning Strategies and Tips
Typical Cloning Strategies and Tips (archive)
Some useful tips and strategies for DNA cloning. (From Mullin's lab at UCSF)
Last update 07-Dec-2007, Rating Fair of 2 votes.
Write your comment
|
By siddartha
on 07-Aug-2008
i want to know the exact meaning of cloning from the basics... I want to know the cloning strategies... |
|
|
By Basha
on 07-Dec-2007
i wanted to clone of 5.7 kb fragment in pGEMT easy vector.eventhough iam getting white colonies there was no insert only vector band was observed. my gene has NdeI at 5' end and hindIII at 3'end so i selected pET23a vector which has the same sites and ligated in 1:3 (vector:insert) but iam not getting waht may be the reason can u plz guide me to obtain clone of my gene of interest |
Please Login or Register to Post
