Restriction Digestion Methods

Protocols of restriciton digestion from Donis-Kellor lab manual.

Restriction Enzyme Digests
Restriction Enzyme Buffers
Restriction Digestion of Plasmid, Cosmid, and Phage DNAs
Manual Method of Restriction Digestion of Human DNA
Restriction Digests of Human Genomic DNA Using the Biomek 1000 Workstation
Preparation of High Molecular Weight Human DNA Restriction Fragments in Agarose Plugs
Preparation and Restriction Digestion of Human Cells in Agarose Beads
Preparation of Bacteriophage Lambda Concatamers for use as Size Standards in Pulsed-Field Gel Electrophoresis
Electroelution of DNA Fragments From Agarose into Dialysis Tubing
Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose.

Last update 12-May-2005, Rating n/a of 0 votes.