PREPARATION OF BACTERIALLY EXPRESSED RECOMBINANT GST-FUSION PROTEINS

Protocol by Helen in Bornstein Group at University of Washington.

Induction of the GST-Fusion Protein (GST-FP)
Batch Purification of the GST-FP using Glutathione (GSH)-Sepharose
Thrombin cleavage of GST-FP to release the recombinant protein
Gel Filtration Purification of the Recombinant Protein

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