Mammalian luciferase reporter assay for Ag-scFv intracellular interaction (archive)
Protocol from Martin Stocks Ph.D. at Iclectus Ltd. It is part of the intracellular antibody capture (IAC) protocol.
After the intracellular scFv have been rigorously characterised in the yeast (and perhaps also the in vitro assays), the ability of these intracellular scFv to bind to their target antigen in mammalian cells must be tested. This can conveniently be assessed by using mammalian reporter assays such as CAT assay or a more sensitive method is the luciferase reporter assay.
Last update 09-May-2005, Rating n/a of 0 votes.
