Gentle lysis of mammalian cells for cytochrome c release assay
Lysis in the sucrose-containing Buffer ("Mito-Buffer") is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the cytosol. (CellDeath.de)
Last update 08-May-2005, Rating n/a of 0 votes.