RNase Protection Assay

Protocol from the Knight lab at Loyola University.

Basic reference: Ausubel,F.M. et al. 1989.Current Protocols in Molecular Biology. John Wiley and sons, N.Y.

Templates are cloned into pGEM vectors (Promega) that contain the bacterial RNA polymerase promotors SP6 and T7 flanking the cloning region. Remember you want to have a probe that is an antisense RNA, thus you have to copy the + DNA strand (coding strand).

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