Linearity of RNase Protection Assays

A technical bulletin article from Ambion on linearity of RNase protection assay. (Ambion).

The amount of protected probe is directly proportional to the amount of target mRNA in the sample. If an internal control probe or synthetic sense strand is included in the experiment, the assay can be used for relative and absolute quantitation, respectively.

Last update 06-May-2005, Rating n/a of 0 votes.