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Biochemistry and molecular biology commonly used buffer, media, solution and reagent

  • 1M Acetic acid
  • NP-40 lysis buffer
  • RIPA lysis buffer
  • 30% acrylamide
  • 40% acrylamide
  • 10M Ammonium acetate
  • Alkaline gel loading buffer
  • 1000x Ampicillin (sodium salt) 100mg/ml
  • 25mg/ml Ampicillin stock solution
  • 10% APS solution
  • 0.5M Ascorbat
  • 0.1M ATP
  • 1M Bis-Tris buffer, pH 7.0
  • 10-25x BLOTTO buffer
  • 2.5M CaCl2
  • 1 M CaCl2 (for competent cell)
  • 34mg/ml Chloramphenical stock solution
  • 1M Citrate, pH 5.0
  • 1x DEPC treated H2O
  • 6x DNA loading dye
  • 1M DTT
  • 0.5 M EDTA (pH 8.0)
  • 10 mg/ml Ethidium bromide
  • 6X Gel loading buffer (bromophenol blue and sucrose)
  • 6X Gel loading buffer with Ficoll
  • 6 X Gel loading buffer with glycerol
  • 6X Gel loading buffer with sucrose
  • 10% Glycerol
  • 1M HCl
  • 2.5N HCl
  • 2 x HEPES-buffered saline
  • 1M IPTG
  • 20% IPTG
  • 25mg/ml Kanamycin stock solution
  • 1x LB medium (Luria-Bertani medium)
  • 1M LiCl
  • Luria Broth
  • 10X M9 salts
  • 1 M Magnesium acetate
  • 1 M MgCl2
  • 10X Microtubules stabilizing buffer (10X MTSB)
  • M9 Minimal Medium
  • 10x MOPS electrophoresis buffer
  • 1x PBS
  • 10x PBS
  • Phenol:chloroform
  • 10 mM Phenylmethylsulfonyl fluoride (PMSF)
  • 1 M Potassium acetate
  • 1x SOC
  • 3M sodium acetate (Na acetate)
  • 20x SSC
  • 20x SSPE
  • 20mg/ml Streptomycin stock solution
  • 1x TAE electrophoresis buffer
  • 10 X TBE (TRIS/BORATE/EDTA)
  • 12.5mg/ml Tetracycline stock solution
  • 50 X Tris-acetate for electrophoresis (TAE)
  • 1M Tris HCl pH 6-8
  • 2% X-gal
  • 1x YT (yeast extraction)
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