Laboratory media reagent and buffer recipes

Biochemistry and molecular biology commonly used buffer, media, solution and reagent

Molar calculation (M): Moles per liter of solution. It refers to the number of grams equal to its molecular weight. e.g. 1M NaCl solution contains 58.456 grams of NaCl in 1 liter water as the MW of NaCl is 58.456g/mole.

Percent solution (%): Weight (W) and volume (V) percent concentration. For W/V percent concentration it means grams (weight) in 100 ml (volume) solution. For V/V percent concentration it means ml (volume) in 100 ml (volume) solution.

"X" (fold) solution: refers to the X folds concentrated solution. e.g. 10X PBS needs to be diluted 10 times to be used as working solution (1x).

Buffer recipe quick finder

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Protein MW kDa:

Weight to mol conversion

g     mg     µg     ng     pg     fg             mol     mmol     µmol     nmol     pmol     fmol     amol

molecules

Mole to Weight converstion

mol     mmol     µmol     nmol     pmol     fmol     amol               g     mg     µg     ng     pg     fg

molecules

Molar concentration calculation:

g     mg     µg     ng     pg     fg     in      l     ml     µl     nl     pl     fl       M     mM     µM     nM     pM     fM     aM

M     mM     µM     nM     pM     fM     aM     in      l     ml     µl     nl     pl     fl       g     mg     µg     ng     pg     fg