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12.5mg/m Tetracycline stock solution
1. Dissolve 1 g tetracycline in 75 ml of 1:1 vol/vol distilled water:ethanol.2. Make up volume to 80 ml with 1:1 vol/vol distilled water:ethanol.
3. Sterilize by filtration and wrap aliquots in aluminium foil and stored at -20 ˇăC.
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