12.5mg/ml Tetracycline stock solution buffer
1 Dissolve 1 g tetracycline in 75 ml of 1:1 vol/vol distilled water:ethanol.
2 Make up volume to 80 ml with 1:1 vol/vol distilled water:ethanol.
3 Sterilize by filtration and wrap aliquots in aluminium foil and stored at -20 C.