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NP-40 lysis buffer

Sodium Chloride ---------------- 150 mM
NP-40 ----------------------- 1%
Tris, pH 8.0 ------------------ 50 mM

NP-40 lysis buffer is one of the most commonly used lysis buffer. NP-40 is a nonionic detergent.
Sometimes Triton X-100 can be used to replace NP-40.