| 1 | Sodium Chloride ---------------- 150 mM |
| 2 | NP-40 ----------------------- 1% |
| 3 | Tris, pH 8.0 ------------------ 50 mM |
| NP-40 lysis buffer is one of the most commonly used lysis buffer. NP-40 is a nonionic detergent. | |
| Sometimes Triton X-100 can be used to replace NP-40. |