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M9 Minimal Medium
1. Add 1 g NH4Cl and 3 g glucose in 100 ml 10X M9 Salts salution.2. Add 1 ml 1 M MgSO4 and 10 ml Gibco MEM vitamin solution.
3. Make up volume to 1 liter with distilled water.
4. Check that the pH is 7.0-7.4.
5. Filter sterilize through a 0.22 micron filter.
6. Add 1 ml sterile 100 mM CaCl2 solution. Store at room temperature.
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