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50 X Tris-acetate for electrophoresis (TAE)

1. Dissolve 242 g Tris base in 800 ml distilled water.
2. Add 57.1 ml glacial acetic acid and 100 ml 0.5 M EDTA (pH 8.0)
3. Make up volume to 1 liter with distilled water.
4. Store at room temperature.