| 1 | Dissolve 380 g acrylamide (DNA-sequencing grade) and 20 g N,N-methylenebisacrylamide. |
| 2 | Add 600 ml distilled water. |
| 3 | Heat to 37C to dissolve acrylamide. Adjust volume to 1 liter with distilled water. |
| 4 | Sterilize by filtration through a 0.45um filter. |