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20x SSPE
175.3 g NaCl27.6 g NaH2PO4?H2O
7.4 g EDTA
in 800 ml of H2O.
Adjust the pH to 7.4 with NaOH. Adjust the volume to 1 liter with H2O. Dispense into aliquots. Sterilize by autoclaving.
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