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10x MOPS electrophoresis buffer

41.8 g MOPS

in 700 ml of sterile DEPC-treated H2O.

Adjust the pH to 7.0 with 2 N NaOH.

Add 20 ml of DEPC-treated 1 M sodium acetate
20 ml of DEPC-treated 0.5 M EDTA (pH 8.0).

Adjust the volume to 1 liter with DEPC-treated H2O.