10X Microtubules stabilizing buffer (10X MTSB) 1. Dissolve 15 G PIPES, 1.9 g EGTA, 1.32 g MgSO4กค7H2O and 5 g KOH in 800 ml distilled water.2. Adjust to pH 7.0 with KOH. 3. Make up volume to 1 liter with distilled water. 4. Sterilize by autoclaving.
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