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10X Microtubules stabilizing buffer (10X MTSB)

1. Dissolve 15 G PIPES, 1.9 g EGTA, 1.32 g MgSO4กค7H2O and 5 g KOH in 800 ml distilled water.
2. Adjust to pH 7.0 with KOH.
3. Make up volume to 1 liter with distilled water.
4. Sterilize by autoclaving.