Biochemistry and molecular biology commonly used buffer, media, solution and reagent

  • 2X YT media

  • 1M Acetic acid

  • NP-40 lysis buffer

  • RIPA lysis buffer

  • 30% acrylamide

  • 40% acrylamide

  • 10M Ammonium acetate

  • Alkaline gel loading buffer

  • 1000x Ampicillin (sodium salt) 100mg/ml

  • 25mg/ml Ampicillin stock solution

  • 10% APS solution

  • 0.5M Ascorbat

  • 0.1M ATP

  • 1M Bis-Tris buffer, pH 7.0

  • 10-25x BLOTTO buffer

  • 2.5M CaCl2

  • 1 M CaCl2 (for competent cell)

  • 34mg/ml Chloramphenical stock solution

  • 1M Citrate, pH 5.0

  • 1x DEPC treated H2O

  • 6x DNA loading dye

  • 1M DTT

  • 0.5 M EDTA (pH 8.0)

  • 10 mg/ml Ethidium bromide

  • 6X Gel loading buffer (bromophenol blue and sucrose)

  • 6X Gel loading buffer with Ficoll

  • 6 X Gel loading buffer with glycerol

  • 6X Gel loading buffer with sucrose

  • 10% Glycerol

  • 1M HCl

  • 2.5N HCl

  • 2 x HEPES-buffered saline

  • 1M IPTG

  • 20% IPTG

  • 25mg/ml Kanamycin stock solution

  • 1x LB medium (Luria-Bertani medium)

  • 1M LiCl

  • Luria Broth

  • 10X M9 salts

  • 1 M Magnesium acetate

  • 1 M MgCl2

  • 10X Microtubules stabilizing buffer (10X MTSB)

  • M9 Minimal Medium

  • 10x MOPS electrophoresis buffer

  • 1x PBS

  • 10x PBS

  • Phenol:chloroform

  • 10 mM Phenylmethylsulfonyl fluoride (PMSF)

  • 1 M Potassium acetate

  • 1x SOC

  • 3M sodium acetate (Na acetate)

  • 20x SSC

  • 20x SSPE

  • 20mg/ml Streptomycin stock solution

  • 1x TAE electrophoresis buffer

  • 10 X TBE (TRIS/BORATE/EDTA)

  • 12.5mg/ml Tetracycline stock solution

  • 50 X Tris-acetate for electrophoresis (TAE)

  • 1M Tris HCl pH 6-8

  • 2% X-gal

  • 1x YT (yeast extraction)