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Technique / Immunology / ELISA assay
| Enzyme-Linked ImmunoSorbent Assay, or ELISA, is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. |
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Enzyme-linked immunosorbent assays. (Curr Protoc Immunol. 2001 May;Chapter 2:Unit 2.1.) Hornbeck P. University of Maryland, Baltimore, Maryland, USA. This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid-phase reactants. In the first four protocols, solid-phase reactants are prepared by adsorbing an antigen or antibody onto plastic microtiter plates; in the next two protocols, the solid-phase reactants are cell-associated molecules. In all protocols, the solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled to an enzyme. Unbound conjugates are washed out and a chromogenic or fluorogenic substrate is added. As the substrate is hydrolyzed by the bound enzyme conjugate, a colored or fluorescent product is generated. Finally, the product is detected visually or with a microtiter plate reader. The amount of product generated is proportional to the amount of analysate in the test mixture. The Support Protocol can be used to optimize the different ELISAs. The second support protocol presents a method for preparing alkaline phosphatase conjugates. Proteins Can Be Detected and Quantitated by Using an Enzyme-Linked Immunosorbent Assay (Biochemistry) I. The Molecular Design of Life 4. Exploring Proteins Figure. Indirect ELISA and Sandwich ELISA (Biochemistry) I. The Molecular Design of Life 4. Exploring Proteins 4.3. Immunology Provides Important Techniques with Which to Investigate Proteins Figure. The principle of the enzyme-linked immunosorbent assay (ELISA) (Immunobiology) Appendix I. Immunologists' Toolbox The detection, measurement, and characterization of antibodies and their use as research and diagnostic tools. Biotinylated Antibodies used in ELISA (Forum) sandwich ELISA for the detection of Protein G in samples 
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Last update: 
09-May-2008 03:00 am
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[Analysis on bioactivity of HIV-1 integrase by ELISA method] Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007 Mar;36(2):179-84
Authors: Feng WH, Huang JS, Zhan JB
OBJECTIVE: To develop an ELISA-based method for analyzing biologic activities of HIV-1 integrase and for high throughput screening of integrase inhibitors. METHODS: After expression, renaturation and purification of integrase, the bioactivity of integrase and the inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and chemiluminescent substrates. RESULT: (1) The specific activity of the purified integrase was 54.92 units/mg of protein. (2)IC(50) (concentration causing 50% inhibition of integrase) of luffin-a was (0.63 +/- 0.026) micromol/L. CONCLUSION: The non-radioactive assay can be used for analysis of bioactivities and high throughput screening of inhibitors of HIV-1 integrase.
[Detection of serum Epstein-Barr virus antibody level by ELISA in normal populations and nasopharyngeal carcinoma patients in Zhongshan City of China] Di Yi Jun Yi Da Xue Xue Bao. 2002 Sep;22(9):817-8, 822
Authors: Cheng MT, Cheng WM, Ji MF, Liang JS
OBJECTIVE: To understand the distribution of Epstein-Barr virus (EBV) antibodies, namely EBNA 1/IgA, ZEBRA/IgA, EBNA 1/IgG in the populations of different ages or genders in Zhongshan City of Guangdong Province, China. METHOD: A total of 484 serum samples were obtained from the population of Zhongshan City including 16 patients with nasopharyngeal carcinoma (NPC) and were assayed with enzyme-linked immunosorbent assay (ELISA) for EBV antibodies. RESULTS: The serum antibody level of EBNA1/IgA, represented by its relative optical density value abbreviated as D(lambda), was 0.726 +/- 0.541 in male and 0.563 +/- 0.340 in female subjects, showing significant difference (P
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