Home Technique / Proteomics and protein biochemistry / Protein Mass Spectrometry


The American Society for Mass Spectrometry (ASMS) protocol
Official web site for Mass Spectrometry research established in 1969. Many valuable news, publications, events etc. ...

Setup of a LC/MS/MS mass spectrometer protocol
Procedures on setting up a nano-scale capillary system, a static nano-spray system and a Mico-spray system. (Michigan State University Proteomics Core) ...

In-gel Tryptic Digest for Protein identification by Mass Spectrometry protocol
David Miyamoto, Michisin lab at Harvard Medical School. ...

Reduction and Alkylation of a Protein protocol
A short protocol on Reduction and Alkylation of a Protein from Molecular structure facility, UC Davis. ...

Sample preparation for Mass Spectrometry analysis new protocol
Useful tips and protocol for protein sample preparation for mass spectrometry analysis. (Protein service lab, Swiss Federal Institute of Technology Zurich) ...

MALD-TOF mass spec protocol new protocol
From PROWL - a resource for protein chemistry and mass spectrometry. (Rockefeller University). Protocols include: 1. MALDI samples preparation Dried droplet Slow crystallization Polycrystalline films Seeded films 2.Endoproteases and exopeptidase ...

Preparing SDS-PAGE Gels for Protein Identification by Mass Spectrometry protocol
Description on before and after protein electrophoresis and post staining for mass spec analysis. (Proteomics core, New York University). ...

Staining of Gels for Mass Spectrometric Analysis new protocol
Protocol of gel staining for mass spec analysis, with figures of EMBL Silver staining, Blum Silver staining and Vorum Silver staining. (NCBA, China) ...

Mass Spectrometry Data Analysis new protocol
The mass spec data analysis demonstration can be downloaded in word doc file. This example demonstrates a number of ways to classify mass spectrometry data and shows some statistical tools that can be used to look for potential disease markers. The data in thi ...

MALDI-TOF mass spectrometry introduction new recommended review
An excellent introduction on Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry by John J. Lennon at University of Washington. ...

Basic mass spectrometry introduction and data interpretation new recommended review
Introduction on basic mass spectrometry and some samples on how you should interpret the MS data. (Organic chemistry online) ...

The principles of MS quantitation review
From IonSource maintained by Andrew Guzzetta. Table of Contents: 1.Introduction to MS Quantitation *Total Ion Current (TIC) * Selected Ion Monitoring (SIM) * Selected Reaction Monitoring (SRM / MRM) 2.Development of an SRM Transition 3.The In ...

What is mass spectrometry (British mass spectrometry society) review
A nice web page introducing mass spectrometry. (British mass spectrometry society) What is Mass Spectrometry What is a Mass Spectrometer? How does a molecule become charged? How does a molecule become charged? What happens next? What types of analys ...

What is mass spectrometry (American society for mass spectrometry) review
A detailed introduction on mass spectrometry from American Society for Mass Spectrometry (ASMS). Index: Why should you be interested in mass spectrometry? Did you know that mass spectrometry is used to... What can mass spectrometry do for you? ...

An introduction to Mass Spectrometry review
An introduction to Mass Spectrometry presented by Department of Biochemistry at University of Washington. Mass spectrometers consist of three essential parts. The first, an ionization source, is a device to convert molecules into gas-phase ions. Two powerfu ...

Isotope-Coded Affinity Tag (ICAT) Analysis Introduction review
An approach to high-throughput quantitative proteome analysis that comprises the following three components: (1) selective labeling of protein mixtures using isotope-coded affinity tag (ICATTM) reagents, (2) multidimensional chromatographic purification and se ...

Mass Spec Protein Identification Principles new review
Within the last few years, mass spectrometry has revolutionized analysis of proteins by providing rapid and sensitive identification of proteins in 1-D and 2-D gels. The principles and features of the protein identification methods by mass spec are described. ...

Proteome analysis by 2D electrophoresis and mass spec: strengths and limitations new review
By Sarka Beranova-Giorgianni, Trends in Analytical Chemistry, Vol. 22, No. 5, 2003 (free accessible review pdf file). Abstract: Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key components of current proteomics t ...

Mass Spectrometry and Biotechnology Resource site
Created and Edited by Andrew Guzzetta. Some sorces include: - Principles of MS Quantitation - Small Molecule Drug Metabolism - Electrospray Mass Spectra Interpretation? Isotopes (tutorial) - Convert to Capillary HPLC (tutorial) - Protein Deamidati ...

Interpreting Electrospray Mass Spectra recommended troubleshooting
An IonSource.Com tutorial on interpreting electrospray mass spectra. This tutorial mainly focuses on the interpretation of mass spectra containing multiply charged molecules generated by the electrospray ionization process. In this tutorial we use peptides ...

Mass Spectrometry Troubleshooting Guide new troubleshooting
The troubleshooting guide on mass specetrometry covers problems on vaccum system, sensitivity and resolution, data analysis. (by Ken Busch, David Bostwick, and Sarah Shealy, School of Chemistry and Biochemistry at Georgia Tech) ...

Mascot Search Database for Mass Spectrometry recommended database
A powerful mass spectrometry analysis and search database. (Matrix Science Inc) Features: Peptide Mass Fingerprint: The experimental data are a list of peptide mass values from an enzymatic digest of a protein. Sequence Query: ...

Mass Spectrometry Software collections from Wiley new software
A collection of Mass Spectrometry softwares and tools including web based tools, freeware and sharewares, commercial softwares from Wiley. ...

Isotope Pattern Calculator v4.0 software
For naturally occurring isotopes version developed by Junhua Yan. ...

PROWL mass spectrometry analysis online tools software
Online tools provided by Rockefeller University's PROWL site. ProFound - a tool for searching a protein sequence database using information from mass spectra of peptide maps. A Bayesian algorithm is used to rank the protein sequences in the database accordi ...

Mass Spectrometry (bio.com InFocus series) media
From bio.com InFocus series (in mp3 or pdf transcript format). Mass Spectrometry is a powerful analytical technique used to identify and elucidate compounds, structures and properties of molecules. ...

More mass spectrometry protocols

Mass spec technique books
(Biowww bookshelf)
mass spectrometry related techniques

Protein Candidates Needed for MS/MS protein identification method development
(Forum)
protein identification with tandem mass spectrometry


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