Home Technique / Cytometry and fluorescence microscopy / Fluorescent confocal microscopy
Immunofluorescence Double Staining Method Parallel Approach
recommended
protocol
Nice protocol on double staining of cells, tissue sections (from IHC world).
The double-immunofluorescence or triple-immunofluorescence technique uses two or three primary antibodies raised from hosts of different species. For example mouse-anti-actin and g ...
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
recommended
protocol
Written by Louise Cramer (actin part)and Arshad Desai (tubulin part), Mitchinson lab at Harvard medical school.
Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of ind ...
Mitochondria staining and Actin Filament double staining protocol
protocol
This mitochondria staining protocol uses MitoTracker green to stain live cell mitochondria on coverslips. It uses Phalloidin Red to stain the fixed cells for visualization of f-actin.
The double staining protocol is from Rob Phillips Group at CIT. ...
General protocol on Immunofluorescence staining
protocol
A general Immunofluorescence staining method (IHC world). ...
Double immunofluorescence staining for BCL-6 and else
new
protocol
Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved (from Giorgio Cattoretti's lab at Columbia University) ...
Fixing and pelleting chromatin/nuclei from extracts onto coverslips for immunofluorescence
protocol
Protocol written by Arshad Desai
in Mitchinson lab at Harvard medical school. ...
IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Vector Red
protocol
By Josiah N. Wilcox and Romesh R. Subramanian From WIlcox lab at Emory University. ...
Anti-HA tag Immunocytology
new
protocol
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California)
Protocol from Yale Genome Analysis Center YGAC. ...
Large-scale Immunocytology
protocol
This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes. Two dishes can be processed simultaneously. The last step is to transfer each sample to a well on a polylysin ...
Double Immunofluorescence GFP/BrdU staining
protocol
A immunofluorescence protocol for double staining of GFP and BrdU. Doc protocol is from Dr.Gordon Fishell lab at New York University School of Medicine. ...
Protocols for Fluorescence Microscopy Samples
protocol
Protocols and buffer recipes on: Autoflourescence
Cell Biology
Buffers
Immunofluorescence
Mounting Media
Protocol from The MMI Research Core Facility, Ohio State University. ...
Fluorescence Microscopy Tutorial
new recommended
review
An excellent primer on fluorescence microscopy from Olympus web site. It covers introduction, advanced techniques, tutorials and troubleshootings. A highly recommended resource for IHC beginners. ...
How does a confocal microscope work?
review
Basics and graphic illustration of confocal microscope. (Dr. Eric R. Weeks) ...
Introduction to Confocal Microscopy
review
An introduction to confocal microscopy from Nikon. It features some interactive tutorials. ...
BD Living Colors FAQ's - Troubleshooting
troubleshooting
Troubleshooting and FAQ (BD biosicence):
Q I have trouble detecting GFP variant expression in transiently transfected cells. What can I do?
Q How can I troubleshoot fluorescent protein expression in yeast?
Q How can I troubleshoot no or low signal o ...
Fluorescence Microscopy Optimization and Troubleshooting
new
troubleshooting
Fluorescence microscopy optimization tips including image brightness, image resolution. It also includes a troubleshooting guidance table. (Olympus) ...
The Bio-Rad Fluorescence Database including fluorochrom spectra selection tool
new recommended
database
The Bio-Rad fluorescence database has been designed to allow the user to superimpose graphical fluorochrome data from various fluorochromes onto a normalised axis.
Both the emission and excitation spectra can be plotted from any fluorochrome in the database ...
More confocal microscopy protocols
(Antibody search engine for all commercially available antibodies from over 300 antibody vendors.)
Last update: 
19-May-2013 11:35 pm
