Technique / Molecular Biology / Recombinant virus and gene therapy

Gene therapy is the insertion of genes into an individual's cells and tissues to treat a disease, and hereditary diseases in which a defective mutant allele is replaced with a functional one.

Production of Adenoviruses of your favourite Gene new protocol
A practical protocol on adenoviruse production in HEK293 cells. (Wolf Lab, Harvard School of Public Health) Step 1: recombination Step 2: transfection 293 cells Step 3: Harvest adenovirus Step 4: Adenovirus amplification ...

A simplified system for generating recombinant adenoviruses new protocol
Tong-Chuan He, Shibin Zhou, Luis T.Da Costa, Jian Yu, Kenneth W. Kinzler, and Bert Vogelstein Howard Hughes Medical Institute & Johns Hopkins Oncology Center Proc. Natl. Acad. Sci. USA 95:2509-2514, 1998 Content: Three ways to obtain AdEasy Componen ...

A simple method for the rapid generation of recombinant adenovirus vectors protocol
RD Anderson et al., Gene Therapy (2000) 7, 1034–1038. Full accessible PDF technical paper. ...

Construction of adenovirus vectors through Cre-lox recombination protocol
NIH Pubmedcentral paper on construction of adenovirus vectors through Cre-lox recombination. S Hardy et al., J Virol. 1997 March; 71(3): 1842–1849.  Abstract    Two barriers prevent adenovirus-based vectors from having wide application. One is the di ...

Adenovirus Production Protocol new protocol
A doc file of recombinant adenovirus production protocol. (Eric Kremer, Vect Euro Train) Content: I) Generation of the recombinantvector genome in a plasmid II) Transfection of Recombinant Genome in transcomplementing cells III) Preparation of ...

Adenovirus General Protocol for Infecting Cells new protocol
Time table and protocol for infecting cell lines using adenovirus. It is downloadable in pdf format. (Vector core at Center for Gene Therapy, University of Michigan) ...

Plaque assays for adenovirus titration protocol
This is a short protocol on titration of recombinant adenovirus by plaque assays. (Morris White Lab, Howard Hughes Medical Institute, Division of Endocrinology, Children's Hospital Boston, Harvard Medical School) ...

Virus Vectors & Gene Therapy: Problems, Promises & Prospects recommended review
Written by David Peel, MBChB Special Study Module Project Report, Department of Microbiology & Immunology, University of Leicester. Content Introduction: Viruses as Vectors 2. Retrovirus Vectors 3. Adenovirus Vectors 4. Adeno-Associated Viru ...

Factors to consider before designing recombinant virus new review
Guidlines on designing adv construction from adenovirus core facility at medical college of Wisconsin. ...

Viral vectors and gene therapy review
Review on application of viral vectors and gene therapy. (Rasul Chaudhry's lab) ...

Three ways to obtain AdEasy components site
Bert V's suggestions on three options to obtain the components for construction AdEasy. ...

Troubleshooting diagram for lentivector production new troubleshooting
A flowchart Troubleshooting production of Lentivectors (Dr Patrick SALMON, Geneva School of Medicine). ...

Troubleshooting lentivector production new troubleshooting
Troubleshooting lentivector production from Tronolab. A brief protocol for lentivirus production is available from the web site (pdf file). ...

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Principles of Retroviral Vector Design
(Retroviruses)
Development and Applications of Retroviral Vectors

Adeno-associated Virus
(Cancer Medicine)
Section 15: Gene Therapy -> 67. Cancer Gene Therapy -> Gene Delivery Systems

Table. Properties of Viruses Used to Derive Gene Transfer Vectors
(Retroviruses)
Development and Applications of Retroviral Vectors Comparison to Other Gene Transfer Systems

Vectors Based on Recombinant Viruses
(Eurekah Bioscience Collection)
Hepatocyte Transplantation and Liver-Directed Gene Therapy -> Liver-Directed Gene Therapy

Adenovirus: structure and pathogenesis etc
(Medical Microbiology)
Section 2. Virology

Figure. Types of gene therapy in mammals.
(An Introduction to Genetic Analysis)
13. Applications of Recombinant DNA Technology Gene therapy

Table. Animal Viruses Commonly Used in Molecular Biology
(Molecular Cell Biology)
6. Manipulating Cells and Viruses in Culture 6.3. Viruses: Structure, Function, and Uses

Viruses: Structure, Function, and Uses
(Molecular Cell Biology )
6. Manipulating Cells and Viruses in Culture

Principles of molecular genetic-based therapies and treatment
(Human Molecular Genetics 2)
22.1. Principles of molecular genetic-based therapies and treatment with recombinant proteins or genetically engineered vaccines

Gene therapy
(Human Molecular Genetics 2)
22. Gene therapy and other molecular genetic-based therapeutic approaches -> 22.1. Principles of molecular genetic-based therapies and treatment with recombinant proteins or genetically engineered vaccines

Adeno-associated Virus
(Cancer Medicine)
Section 15: Gene Therapy -> 67. Cancer Gene Therapy -> Gene Delivery Systems