Technique / Molecular Biology / Recombinant virus and gene therapy
| Gene therapy is the insertion of genes into an individual's cells and tissues to treat a disease, and hereditary diseases in which a defective mutant allele is replaced with a functional one. |
Production of Adenoviruses of your favourite Gene
new
protocol
A practical protocol on adenoviruse production in HEK293 cells. (Wolf Lab, Harvard School of Public Health)
Step 1: recombination
Step 2: transfection 293 cells
Step 3: Harvest adenovirus
Step 4: Adenovirus amplification ...
A simplified system for generating recombinant adenoviruses
new
protocol
Tong-Chuan He, Shibin Zhou, Luis T.Da Costa, Jian Yu,
Kenneth W. Kinzler, and Bert Vogelstein
Howard Hughes Medical Institute & Johns Hopkins Oncology Center
Proc. Natl. Acad. Sci. USA 95:2509-2514, 1998
Content:
Three ways to obtain AdEasy Componen ...
A simple method for the rapid generation of recombinant adenovirus vectors
protocol
RD Anderson et al., Gene Therapy (2000) 7, 1034–1038. Full accessible PDF technical paper.
...
Construction of adenovirus vectors through Cre-lox recombination
protocol
NIH Pubmedcentral paper on construction of adenovirus vectors through Cre-lox recombination.
S Hardy et al., J Virol. 1997 March; 71(3): 1842–1849.
Abstract
Two barriers prevent adenovirus-based vectors from having wide application. One is the di ...
Adenovirus Production Protocol
new
protocol
A doc file of recombinant adenovirus production protocol. (Eric Kremer, Vect Euro Train)
Content:
I) Generation of the recombinantvector genome in a plasmid
II) Transfection of Recombinant Genome in transcomplementing cells
III) Preparation of ...
Adenovirus General Protocol for Infecting Cells
new
protocol
Time table and protocol for infecting cell lines using adenovirus. It is downloadable in pdf format. (Vector core at Center for Gene Therapy, University of Michigan) ...
Plaque assays for adenovirus titration
protocol
This is a short protocol on titration of recombinant adenovirus by plaque assays. (Morris White Lab, Howard Hughes Medical Institute, Division of Endocrinology, Children's Hospital Boston, Harvard Medical School) ...
Virus Vectors & Gene Therapy: Problems, Promises & Prospects
recommended
review
Written by David Peel, MBChB Special Study Module Project Report, Department of Microbiology & Immunology, University of Leicester.
Content
Introduction: Viruses as Vectors
2. Retrovirus Vectors
3. Adenovirus Vectors
4. Adeno-Associated Viru ...
Factors to consider before designing recombinant virus
new
review
Guidlines on designing adv construction from adenovirus core facility at medical college of Wisconsin. ...
Viral vectors and gene therapy
review
Review on application of viral vectors and gene therapy. (Rasul Chaudhry's lab) ...
Three ways to obtain AdEasy components
site
Bert V's suggestions on three options to obtain the components for construction AdEasy. ...
Troubleshooting diagram for lentivector production
new
troubleshooting
A flowchart Troubleshooting production of Lentivectors (Dr Patrick SALMON, Geneva School of Medicine). ...
Troubleshooting lentivector production
new
troubleshooting
Troubleshooting lentivector production from Tronolab.
A brief protocol for lentivirus production is available from the web site (pdf file). ...
(Retroviruses)
Development and Applications of Retroviral Vectors
(Cancer Medicine)
Section 15: Gene Therapy -> 67. Cancer Gene Therapy -> Gene Delivery Systems
(Retroviruses)
Development and Applications of Retroviral Vectors Comparison to Other Gene Transfer Systems
(Eurekah Bioscience Collection)
Hepatocyte Transplantation and Liver-Directed Gene Therapy -> Liver-Directed Gene Therapy
(An Introduction to Genetic Analysis)
13. Applications of Recombinant DNA Technology Gene therapy
(Molecular Cell Biology)
6. Manipulating Cells and Viruses in Culture 6.3. Viruses: Structure, Function, and Uses
(Molecular Cell Biology )
6. Manipulating Cells and Viruses in Culture
(Human Molecular Genetics 2)
22.1. Principles of molecular genetic-based therapies and treatment with recombinant proteins or genetically engineered vaccines
(Human Molecular Genetics 2)
22. Gene therapy and other molecular genetic-based therapeutic approaches -> 22.1. Principles of molecular genetic-based therapies and treatment with recombinant proteins or genetically engineered vaccines
(Cancer Medicine)
Section 15: Gene Therapy -> 67. Cancer Gene Therapy -> Gene Delivery Systems
Last update: 
01-Mar-2010 11:41 pm
Gene therapy for Parkinson's disease.
Mov Disord. 2010 Feb 25;25(S1):S161-S173 Authors: Bjorklund T, Kordower JHGenetic basis and gene therapy trials for thyroid cancer.
Cancer Genomics Proteomics. 2010 Jan-Feb;7(1):31-49 Authors: Al-Humadi H, Zarros A, Al-Saigh R, Liapi CNew aspects in vascular gene therapy.
Curr Opin Pharmacol. 2010 Feb 15; Authors: Karvinen H, Ylä-Herttuala SFuture prospects and challenges of anti-angiogenic cancer gene therapy.
Hum Gene Ther. 2010 Feb 17; Authors: Samaranayake H, Määttä AM, Pikkarainen J, Yla-Herttuala S