Genetics / Cytogenetics analysis protocols and methods

Technique / Genetics / Cytogenetics analysis

Cytogenetics is a branch of genetics that is concerned with the study of chromosomes and cell division. It includes routine analysis of G-Banded chromosomes, other cytogenetic banding techniques, as well as molecular cytogenetics such as fluorescent in situ hybridization (FISH) and comparative genomic hybridization (CGH).

Comparative Genomic Hybridization (CGH) new protocol
CGH is a molecular cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels. Laboratory of Autom ...

Cytogenetic protocols (The Jackson Laboratory) protocol
General Cytogenetic Protocols Blood Culture Meiotic Preparations from Testis Mitotic Chromosome Preparations from Bone Marrow Mitotic Chromosome Preparations from Fetal Liver Mitotic Chromosome Preparations from Embryo Tail Tips Mitotic Chromosome Prep ...

Cytogenetics information site new protocol
Some cytogenetics/Fish information and image examples. From Seattle, WA Dynagene Cytogenetics Laboratory. ...

In vitro micronuclei assay (MN assay) new review
Micronuclei assay (MN assay) is an in vitro assay to detect the mutagenic chemicals that can induce formation of micronuclei in the cytoplasm of interphase cells by interfering the chromosomes structure and segregation. Micronuclei can also be induced by radi ...

Cytogenetic Resources site
Cytogenetic Resources from Genetics Education Center, University of Kansas Medical Center. URL: Cytogenetic Resources Contents: links ...

comparative genomic hybridization (CGH) site
Protocol from Institut f¨¹r Pathologie "Rudolf-Virchow-Haus" University Hospital Charit¨¦ Schumannstr. 20/21 10117 Berlin CGH is a molecular cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DN ...

Cytogenetics Lab Protocols site
UW Waisman Center Cytogenetics Lab Protocols including Culture and Harvest , Staining, Reagent Preparation. ...

FISH Guide and Troubleshooting (Tavi) new troubleshooting
By Octavian Henegariu at Yale University. This general and troubleshooting guid gives very detailed description on practical application of fluorescent in situ hybridization. ...

Banding patterns of human chromosomes
(Human Molecular Genetics)
2 Appendix Supplementary information

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Last update:

08-May-2008 04:34 pm

Related new papers and reviews

Evolutionary cytogenetics in salamanders.

Salamanders (Amphibia: Caudata/Urodela) have been the subject of numerous cytogenetic studies, and data on karyotypes and genome sizes are available for most groups. Salamanders show a more-or-less distinct dichotomy between families with large chromosome numbers and interspecific variation in chromosome number, relative size, and shape (i.e. position of the centromere), and those that exhibit very little variation in these karyological features. This dichotomy is the basis of a major model of karyotype evolution in salamanders involving a kind of 'karyotypic orthoselection'. Salamanders are also characterized by extremely large genomes (in terms of absolute mass of nuclear DNA) and extensive variation in genome size (and overall size of the chromosomes), which transcends variation in chromosome number and shape. The biological significance and evolution of chromosome number and shape within the karyotype is not yet understood, but genome size variation has been found to have strong phenotypic, biogeographic, and phylogenetic correlates that reveal information about the biological significance of this cytogenetic variable. Urodeles also present the advantage of only 10 families and less than 600 species, which facilitates the analysis of patterns within the entire order. The purpose of this review is to present a summary of what is currently known about overall patterns of variation in karyology and genome size in salamanders. These patterns are discussed within an evolutionary context.