Home Technique / Molecular Biology / Gene targeting

b-gal GFP luciferase reporter assay system (3)

Basic mouse techniques (5)

ES cells gene targeting (4)

Inducible tetracycline cre-loxP system (1)

principle of phenotype characterization (2)


C elegans Gene Knockout Protocols new recommended protocol
Collection of protocols on C.elegans gene knockout by Leon Avery at The University of Texas Southwestern Medical Center at Dallas. One-Step Knockout with Chemical Mutagens: Reverse Genetics: Isolating Deletions in PCR Screens of Mutagenized Populations fro ...

Gene targeted mice production protocol
A technical mini-review on how to generate gene targeting mice (University of Wisconsin Biotechnology Center). Content: 1. construction of targeting vector containing regions of identity with the mouse chromosome (homology units or arms), a sele ...

Gene knockout mice database protocol
Gene knockout mice database contains data regarding the phenotypes rendered by the knockout of various molecules. GENE KNOCKOUTS WHICH ARE COMPATIBLE WITH VIABILITY GENE KNOCKOUTS WHICH RESULT IN PRENATAL MORTALITY GENE KNOCKOUTS WHICH RES ...

Generating conditional transgenic mice by a tetracycline-responsive promoter new protocol
This is the original technical paper for conditional transgenic mouse generation using tetracycline regulated gene expression technique. Proc Natl Acad Sci U S A. 1994 September 27; 91(20): 93029306. Temporal control of gene expression in transgenic mice ...

C.elegans Gene Knockout Protocol by TMP/UV Method protocol
By Hitoshi Inada and Ikue Mori, Laboratory of Molecular Neurobiology, Division of Biological Science, Graduate School of Science, Nagoya University. The gene knockout is one of the useful approaches to analyze the gene function. In C. elegans, the construct ...

C.elegans Gene Knockout with Conventional Mutagens protocol
By Leon Avery, Ph.D. University of Texas Southwestern Medical Center. ...

The Anatomy of the Laboratory Mouse new hot recommended review
Margaret J. Cook M.R.C. Laboratory Animals Centre Carshalton, Surrey, England Academic Press 1965 Adapted for the Web by: Mouse Genome Informatics The Jackson Laboratory Bar Harbor, Maine May 2005 This book, which has long been out of print, i ...

Homologous Recombination Gene Targeting in Mice new review
A flowchart on Homologous Recombination Gene Targeting in Mice. From Transgenic core facility at University of Virginia. ...

Transgenesis Trouble Shooting Section troubleshooting
Since many people have run into difficulty getting the transgenic procedure working well in their labs, I have decided to add a transgenesis trouble shooting section. This section ... ... URL: ...

Database of Gene Knockout new database
In this section, data regarding the phenotypes rendered by the knockout of various molecules are presented. (FRONTIERS IN BIOSCIENCE) GENE KNOCKOUTS WHICH ARE COMPATIBLE WITH VIABILITY GENE KNOCKOUTS WHICH RESULT IN PRENATAL MORTALITY GENE K ...

More gene targeting protocols

Figure. Double replacement gene targeting can be used to introduce subtle mutations
(Human Molecular Genetics 2)
21. Genetic manipulation of animals 21.3. Use of mouse embryonic stem cells in gene targeting and gene trapping

Figure. Gene targeting using the Cre-loxP recombination system
(Human Molecular Genetics 2)
21. Genetic manipulation of animals 21.3. Use of mouse embryonic stem cells in gene targeting and gene trapping

Figure. Chromosome engineering can be accomplished using cre-loxP systems
(Human Molecular Genetics 2)
21. Genetic manipulation of animals 21.3. Use of mouse embryonic stem cells in gene targeting and gene trapping

Figure. Gene targeting by homologous recombination
(Human Molecular Genetics 2)
21. Genetic manipulation of animals 21.3. Use of mouse embryonic stem cells in gene targeting and gene trapping

Mouse toe number scheme
(Fero lab)
Mouse toe number scheme for managing transgenic mouse.


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