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Technique / Proteomics and protein biochemistry / Protein modifications glycoconjugates phosphorylation
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gel separation of phospho and dephosphoprotein (Forum) Protein phosphorylation separation and detection in vivo ubiquitination assay (Forum) transfect cells with the plasmids expressing my interested protein and HA-ub 
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Last update: 
09-May-2008 07:55 am
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Acquired obesity and poor physical fitness impair expression of genes of mitochondrial oxidative phosphorylation in monozygotic twins discordant for obesity. Am J Physiol Endocrinol Metab. 2008 May 6;
Authors: Mustelin L, Pietilainen KH, Rissanen A, Sovijarvi AR, Piirila P, Naukkarinen J, Peltonen L, Kaprio J, Yki-Jarvinen H
Defects in expression of genes of oxidative phosphorylation in mitochondria have been suggested to be a key pathophysiological feature in familial insulin resistance. We examined whether such defect can arise from lifestyle-related factors alone. Fourteen obesity-discordant (BMI difference 5.2 +/- 1.8 kg/m(2)) and 10 concordant (1.0 +/- 0.7 kg/m(2)) MZ twin pairs aged 24-27 yr were identified among 658 MZ pairs in the population-based FinnTwin16 study. Whole body insulin sensitivity was measured using the euglycemic hyperinsulinemic clamp technique. Transcript profiles of mitochondrial genes were compared using microarray data of fat biopsies from discordant twins. Body composition of twins was determined using DEXA and maximal oxygen uptake (VO2max) and working capacity (Wmax) using a bicycle ergometer exercise test with gas exchange analysis. The obese co-twins had lower insulin sensitivity than their non-obese counterparts (M-value 6.1 +/- 2.0 mg/kgLBM.min vs. 9.2 +/- 3.2 mg/kgLBM.min, P
Phosphopeptide mapping and identification of phosphorylation sites. Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.9
Authors: Meisenhelder J, Hunter T, van der Geer P
Protein phosphorylation is a common modification for many proteins in the cell. Phosphorylation can affect localization of a protein, its stability, and its ability to dimerize or form stable complexes with other molecules. To understand the underlying mechanisms behind the phosphorylation of a given protein, it is often necessary to precisely identify which amino acid residues are phosphorylated. This unit describes the technique of phosphopeptide mapping. In this procedure, a radiolabeled protein is proteolytically digested, and the resulting phosphopeptides are separated in two dimensions on a TLC plate. The phosphopeptides are also analyzed by HPLC and mass spectrometry or peptide microsequencing. Such mapping gives information about the number of phosphorylation sites present in the protein, and can also be used to find out if sites of phosphorylation on a protein change upon treatment of cells with specific agents.
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