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Protein phosphorylation protocols
new recommended
protocol
Protocols for studying protein phosphorylation. Protocols are provided by Invitrogen as the free online version of current protocols in immunology.
* Preparation and Analysis of Phosphorylated Proteins
* Immune-Complex Assays for Tyrosine Prote ...
Phosphoamino acid analysis Mark Kamps method
new
protocol
This is a 32P labeling 2D PAGE gel analysis method from Bart's Cookbook. (Sefton Lab, Salk institute) ...
Phosphoamino acid analysis of non-TCA precipitable proteins
protocol
A short protocol for phosphoamino acid analysis of non-TCA precipitable proteins. ( Jim Woodgett lab, Ontario Cancer Institute/Princess Margaret Hospital)
...
A systematic approach to the analysis of protein phosphorylation
new recommended
review
A free pdf technique paper published on Nature Biotechnol 2001 issue 19.
Nat Biotechnol. 2001 Apr;19(4):375-8.
A systematic approach to the analysis of protein phosphorylation.
Zhou H, Watts JD, Aebersold R.
Abstract:
Reversible protein pho ...
Protein phosphorylation detection by mass spectrometry
review
A brief overview of protein phosphorylation detection by mass spectrometry. (W.M. Keck Facility, Yale University)
In most instances our general approach to identifying sites of protein phosphorylation begins with an in gel trypsin digest of the Coomassie Bl ...
The Protein Kinase Resource
new recommended
site
The Protein Kinase Resource (PKR) aims to become a web accessible compendium of information on the protein kinase family of enzymes. This resource will include tools for structural and computational analyses as well as links to related information maintained b ...
Glycosylation
troubleshooting
A question regarding glycosylation of proteins and how that looks in a
western. I have two different antibodies raised against the same protein. When
I use these on a western, in one case I get a tight band while (antiboby 1) and
in the other I get a ...
More protein phosphorylation protocols
(Forum)
Protein phosphorylation separation and detection
(Forum)
transfect cells with the plasmids expressing my interested protein and HA-ub
