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5' and 3' RACE (4)

Clony PCR (1)

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High fidelity long PCR (5)

In situ PCR (3)

Inverse PCR (2)

Methylation specific PCR (1)

Multiplex PCR (2)

Nested PCR (1)

Overlapping PCR (1)

PCR primer design (9)

PCR purification (3)

Quantitative real-time PCR (18)

RT-PCR (2)

Standard PCR (14)

Touchdown PCR (1)


More pcr protocols

Application of Real-time Polymerase Chain Reaction RT-PCR (Technique review)
(Hongbao Ma et.al., Michigan State University)
Abstract: The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-time version of quantitative polymerase chain reaction (qPCR), itself a modification of polymerase chain reaction (PCR). The procedure of RT-PCR follows the regular PCR procedure, but the DNA is quantified after each round of amplification. Two common methods of quantification are the use of fluorescent dyes that intercalate with double-strand DNA, and modified DNA oligonucleotide probes that fluoresce when hybridized with a complementary DNA. RT-PCR could be combined with reverse transcription polymerase chain reaction to quantify messenger RNA (mRNA) at a particular time for in a particular cell or tissue type. [The Journal of American Science. 2006;2(3):1-15].


(Reviews on PCR methods and techniques)
1: Rumsby G.
An introduction to PCR techniques.
Methods Mol Biol. 2006;324:75-89. Review.

2: Zhang C, Xu J, Ma W, Zheng W.
PCR microfluidic devices for DNA amplification.
Biotechnol Adv. 2006 May-Jun;24(3):243-84. Epub 2005 Dec 2. Review.

3: Kraytsberg Y, Khrapko K.
Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic
mutations.
Expert Rev Mol Diagn. 2005 Sep;5(5):809-15. Review.

4: Adler M.
Immuno-PCR as a clinical laboratory tool.
Adv Clin Chem. 2005;39:239-92. Review.

5: Lauerman LH.
Advances in PCR technology.
Anim Health Res Rev. 2004 Dec;5(2):247-8. Review.

6: Niemeyer CM, Adler M, Wacker R.
Immuno-PCR: high sensitivity detection of proteins by nucleic acid
amplification.
Trends Biotechnol. 2005 Apr;23(4):208-16. Review.

7: Ridgwell K.
Genetics tools: PCR and sequencing.
Vox Sang. 2004 Jul;87 Suppl1:6-12. Review.

8: Mike Makrigiorgos G.
PCR-based detection of minority point mutations.
Hum Mutat. 2004 May;23(5):406-12. Review.

9: Radstrom P, Knutsson R, Wolffs P, Lovenklev M, Lofstrom C.
Pre-PCR processing: strategies to generate PCR-compatible samples.
Mol Biotechnol. 2004 Feb;26(2):133-46. Review.

10: Kato K.
Adaptor-tagged competitive PCR: study of the mammalian nervous system.
C R Biol. 2003 Oct-Nov;326(10-11):941-7. Review.

11: Pohl G, Shih IeM.
Principle and applications of digital PCR.
Expert Rev Mol Diagn. 2004 Jan;4(1):41-7. Review.

12: Stirling D.
Qualitative and quantitative PCR: a technical overview.
Methods Mol Biol. 2003;226:181-4. Review.

13: Wendland J.
PCR-based methods facilitate targeted gene manipulations and cloning
procedures.
Curr Genet. 2003 Nov;44(3):115-23. Epub 2003 Aug 19. Review.

14: Kricka LJ, Wilding P.
Microchip PCR.
Anal Bioanal Chem. 2003 Nov;377(5):820-5. Epub 2003 Aug 19. Review.

15: Dickerson SK, Papavasiliou FN.
A modified digestion-circularization PCR (DC-PCR) approach to detect
hypermutation-associated DNA double-strand breaks.
Ann N Y Acad Sci. 2003 Apr;987:135-9. Review.

16: Hoorfar J, Cook N.
Critical aspects of standardization of PCR.
Methods Mol Biol. 2003;216:51-64. Review.

17: Sachse K.
Specificity and performance of diagnostic PCR assays.
Methods Mol Biol. 2003;216:3-29. Review.

18: Zhu J.
Use of PCR in library screening. An overview.
Methods Mol Biol. 2002;192:353-8. Review.

19: Hui EK, Wang PC, Lo SJ.
PCR-based strategies to clone unknown DNA regions from known foreign
integrants. An overview.
Methods Mol Biol. 2002;192:249-74. Review.

20: Shen B.
PCR approaches to DNA mutagenesis and recombination. An overview.
Methods Mol Biol. 2002;192:167-74. Review.

21: Guo B, Bi Y.
Cloning PCR products. An overview.
Methods Mol Biol. 2002;192:111-9. Review.

22: Gustin K, Burk RD.
PCR-directed linker scanning mutagenesis.
Methods Mol Biol. 2000;130:85-90. Review.

23: Millican DS, Bird IM.
Preparation of single-stranded antisense cDNA probes by asymmetric PCR.
Methods Mol Biol. 1998;105:337-50. Review.

24: Schuler GD.
Electronic PCR: bridging the gap between genome mapping and genome sequencing.
Trends Biotechnol. 1998 Nov;16(11):456-9. Review.

25: Felix CA, Jones DH.
Panhandle PCR: a technical advance to amplify MLL genomic translocation
breakpoints.
Leukemia. 1998 Jun;12(6):976-81.

26: Hooft van Huijsduijnen R.
PCR-assisted cDNA cloning: a guided tour of the minefield.
Biotechniques. 1998 Mar;24(3):390-2. Review.

Books on PCR, RT-PCR and real-time quantitative PCR
(Biowww bookshelf)
PCR related technique books

Purification of small (100bp) PCR products
(Forum)
Small PCR fragment purification discussion

Non-specific PCR products
(Forum)
Non-specific bands in PCR products

fainting of PCR amplification bands with time
(Forum)
PCR products degradation

Non-specific PCR bands
(Forum)
Question on non-specific PCR amplification

Reproducibility of PCR
(Forum)
PCR troubleshooting

RFLP PCR no amplification
(Forum)
PCR no amplification troubleshooting

problems with housekeepings in real-time pcr
(Forum)
house-keeping gene for real-time PCR internal control

Site directed mutagenesis with PCR
(Forum)
PCR mutagenesis method discussion

Sequencing PCR Products - Please Help
(Forum)
PCR products for DNA sequencing


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