Home Technique / Proteomics and protein biochemistry / Chromatography HPLC purification
FPLC fast performance liquid chromatography protocol
new
protocol
The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting.
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Protocol for Packing a Reversed-Phase Microcapillary Column
protocol
By Hookeun Lee, Proteomecenter.org. ...
MBP-fusion protein chromatography purification protocol version 2.0
new
protocol
A very detailed protocol of using chromatography and FPLC to purify MBP-fusion protein in pdf file written by Ken Johnson, Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisco ...
The Chrom-Ed Chromatography Series
new recommended
review
An online chromatography book edited by Dr. R. P. W. Scott.
Content:
Principles and Practice of Chromatography
Introduction
The Development Process
Chromatography Nomenclature
Factors Controlling Retention
Factors Affecting the Magnitude ...
High Performance Liquid Chromatography HPLC: A Users Guide
new
review
A user guide on HPLC from Analytical Spectroscopy Research Group, Advanced Science and Technology Center at University of Kentucky.
Content:
About this Guide
Applications
Bibliography
History
Manufacturers
Technical Assistance
T ...
Chromatography data system (CDS)
new
review
Chromatography data system (CDS)
Acquisition of chromatography data system CDS
Integration of chromatography data system CDS
Identification of chromatography data system CDS
Calib ...
metall ion affinity purification of proteins
troubleshooting
are there other (competitive/better) methods for purifying proteins
using affinity to chelated metal ions than his6 / nickel? ... ...
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source for simple chromatography columns
troubleshooting
Could anyone please point me to a cheap source for plasic (poly ethylene or similar) columns suitable for 1 to 5 ml of charged agarose? ... ...
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HPLC Trouble Shooting
troubleshooting
HPLC Trouble Shooting guide from Thomson Instrument Company.
Abnormal Peak Shape
Fluctuation in Elution time
Fluctuation in Baseline
Noise in Baseline
Rise in Pressure
... ...
URL: ...
HPLC Interactive troubleshooting guide from Varian
new
troubleshooting
Varian inc. Troubleshooting include:
Problems with Baselines
Problems with Clogged Tubing
Problems with Leaks
Problems with Peaks
Problems with Pressure
Column Use &Care Data
Column Washing
Sorbent Specifications ...
An interactive troubleshooting guide on High Performance Liquid Chromatography
troubleshooting
Some covered problems:
1.Peaks
Broad peaks
Ghost peaks
Negative peaks
Peak doubling
Peak fronting
Peak tailing
Spikes
2.Leaks
Leak at column or fittings
Leak at detector
Leak at injection valve
Leak at pump
3.Recovery
Poor sample recovery ...
Downloadable HPLC Troubleshooting
hot
troubleshooting
From MACHEREY-NAGEL Inc.
Content:
chapter 1
broad peaks
fronting
tailing
chapter 2
double peaks
negative peaks
spikes
ghost peaks
chapter 3
lack of sensitivity
poor sample recovery
high back pressure
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LC Troubleshooting guide
troubleshooting
From VWR international Ltd. ...
HPLC troubleshooting guide from Waters
troubleshooting
This is a 68 pages PDF troubleshooting brochure from Waters. ...
HPLC Troubleshooting Guide from Supelco.
troubleshooting
20 pages PDF troubleshooting guide from Supleco, Sigma-Aldrich. ...
Chromatography Equations
software
Some useful general, GC, TLC equations. (from VMR interanational) ...
pKa of Common acids and Common Bases
software
pKa of Common acids and Common Bases (from VMR international). ...
Free webcast of HPLC Troubleshooting from PE
media
Webcast supplied by PE. You will have to register to access the webcasting content.
HPLC Troubleshooting (Part 2 of 2*): Recognizing diagnostic clues and interpreting chromatographic results
Duration: 117 minutes ...
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Affinity Chromatography Protocol for Anitbody Purification.
(Forum)
RP-HPLC purification of 22 aa peptide synthesized by solid-phase synthesis.
Last update: 
19-May-2013 11:34 pm
