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Technique / Proteomics and protein biochemistry / Chromatography HPLC purification
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Antibody Purification by Affinity Chromatography Protocol () Affinity Chromatography Protocol for Anitbody Purification. Problem with RP-HPLC purification of peptide (Forum) RP-HPLC purification of 22 aa peptide synthesized by solid-phase synthesis. 
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Last update: 
11-May-2008 09:03 pm
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Extending the molecular application range of gas chromatography. J Chromatogr A. 2008 Mar 14;1184(1-2):43-60
Authors: Kaal E, Janssen HG
Gas chromatography is an important analytical technique for qualitative and quantitative analysis in a wide range of application areas. It is fast, provides a high peak capacity, is sensitive and allows combination with a wide range of selective detection methods including mass spectrometry. However, the application area of GC is limited because the molecules to be analysed have to be thermally stable and sufficiently volatile. Numerous molecules do not meet these requirements and hence are not amenable to direct GC analysis. Recent research has resulted in better chromatographic columns and methods for sample preparation that enable a significant expansion of the molecular application range of GC. The strategies exploited include conversion of (macro)molecules into smaller species and approaches to reduce the polarity of molecules. In this review we identify four generic routes for extending the applicability of GC. These include high-temperature GC, derivatisation, pyrolysis and thermochemolysis. The principles, recent developments and future perspectives of these routes are discussed and examples of applications using the different options will be shown. Life sciences, metabonomics and profiling strategies for sample characterization are identified as important future drivers for the continued development of GC.
Ion mobility spectrometry detection for gas chromatography. J Chromatogr A. 2008 Jan 4;1177(1):12-27
Authors: Kanu AB, Hill HH
The hyphenated analytical method in which ion mobility spectrometry (IMS) is coupled to gas chromatography (GC) provides a versatile alternative for the sensitive and selective detection of compounds after chromatographic separation. Providing compound selectivity by measuring unique gas phase mobilities of characteristic analyte ions, the separation and detection process of gas chromatography-ion mobility spectrometry (GC-IMS) can be divided into five individual steps: sample introduction, compound separation, ion generation, ion separation and ion detection. The significant advantage of a GC-IMS detection is that the resulting interface can be tuned to monitor drift times/ion mobilities (as a mass spectrometer (MS) can be tuned to monitor ion masses) of interest, thereby tailoring response characteristics to fit the need of a given separation problem. Because IMS separates ions based on mobilities rather than mass, selective detection among compounds of the same mass but different structures are possible. The most successful application of GC-IMS to date has been in the international space station. With the introduction of two-dimensional gas chromatography (2D-GC), and a second type of mobility detector, namely differential mobility spectrometry (DMS), GC prior to mobility measurements can now produce four-dimensional analytical information. Complex mixtures in difficult matrices can now be analyzed. This review article is intended to provide an overview of the GC-IMS/DMS technique, recent developments, significant applications, and future directions of the technique.
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