Home Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec
silver staining protocol from Breeden lab
protocol
silver staining protocol from Breeden lab ...
Silver staining of SDS-PAGEs
new
protocol
Protocol from Bjorkerman lab at CIT (pdf). ...
Reduction/Alkylation and Proteolysis of Electrophoretically Separated Proteins
protocol
The pdf version of the protocol is also available on the web page.
Protocol from The Ludwig Institute for Cancer Research. ...
Precautions on gel preparation and staining
new
protocol
OHSU Proteomics Core Laboratory guidline on gel staining. Some specific gel (coomassie, silver, sypro orange/red/ruby staining) protocols are listed in this page. ...
Trypsin In-gel Digestion Procedure
protocol
S. Chen 7/25/03 (Proteomecenter.org PDF file). ...
Sypro Ruby Staining Protocol
protocol
PDF protocol from Michigan State University Proteomic Core Facility. ...
Tryptic digestion directly in polyacrylamide gel slices
protocol
This protocol is designed for the direct digestion of proteins in Coomassie stained polyacrylamide gels. Direct digestion in the gel band eliminates transfer of the protein of interest to a membrane and allows Coomassie staining to be used. (Biomolecular Resea ...
CNBr Digestion
protocol
A short protocol on CNBr Digestion. (Molecular structure facility, UC Davis). ...
Silver Staining (and De-Staining) of Polyacrylamide Gels
new
protocol
A very detailed procedure on Silver Staining (and De-Staining) of Polyacrylamide Gels including buffer preparation. (Molecular structure facility, UC Davis). ...
Acrylamide gel protein detection methods
protocol
Brief protocols for acrylamide gel protein detection methods including coomassie blue R-250, G-250, silver staining, fluorescent detection and Ruthenium II method. (University of Missouri - Columbia Proteomics Center) ...
Solution Digest with embedded excel calculations
protocol
Original In Solution Digest protocol in ms-word doc from Mike Myers (Cold Spring Harbor) modified by Brett Phinney (Michigan State University). It includes a excel calculator to help you calculate digestion buffer. ...
Rapid ethanol-based Coomassie Blue staining of SDS-polyacrylamide gels
protocol
A staining protocol using ethanol instead of methanol from NWFSC/Molecular Biology Protocols. It also works well with methanol-based stain and destain, but significant amounts of methanol vapor are released into the laboratory---the ethanol is safer and just a ...
Coomassie Stain Protocol
protocol
A nice protocol from Mass Spectrometry core facility at Ohio State University (in MS-word doc format).
The gel must be fixed prior to staining by a non-modifying, precipitation procedure such as the ethanol (or methanol)-acetic acid method used here. If th ...
What if silver stain does not work?
troubleshooting
Our PAGE gels Sometimes stain completely and you get
a "chrome-plated" surface with no trace of DNA bands. This happens with
different operators and everyone keeps his/her own stock solutions. The
AgNO3 we have is a little old, and so is the nonbindin ...
Coomassie Blue: destaining
troubleshooting
I am using PhastGel BlueR coomassie blue dye from Pharmacia, I am concearned
with destaining the gel without losing the signal from the bands (I have
around 10ng protein per band)
I am currently destaining in 10% Acetic acid, this takes quite a ...
PAGE gel staining and troubleshooting
new
troubleshooting
This protocol and troubleshooting guide gives some troubleshooting on silver staining of polyacrylamide gel. (Frank lab, Oklahoma Medical Research Foundation)
1. Gray or brown precipitate appearing as smudges or swirling on gel surface. Bands may be faint o ...
Last update: 
19-May-2013 11:34 pm
