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Chromatography HPLC purification (18)

Conventional protein interaction study (4)

Immunoprecipitation (17)

Protein Array Analysis (8)

Protein assay (2)

Protein electrophoresis (26)

Protein expression, protein extraction and protein purification (44)

protein gel staining and protein digestion for mass spec (16)

Protein Mass Spectrometry (26)

Protein modifications glycoconjugates phosphorylation (7)

Protein oligos desalting concentration and dialysis (8)

Protein-protein interactions (21)

Western blot assay (14)

More proteomics protocols

Overview of proteome analysis.
(Curr Protoc Protein Sci. 2003 Feb;Chapter 22:Unit 22.1.)
Ali-Khan N, Zuo X, Speicher DW.

The Wistar Institute, Philadelphia, Pennsylvania, USA.

This unit reviews the new discipline of proteomics, which includes any
large-scale protein-based systematic analysis of the proteome or defined
sub-proteome from a cell, tissue, or entire organism. Proteomics originated in
the mid-1990 s due to two key enabling advances, availability of complete genome
sequences, and mass spectrometry advances that allowed high sensitivity
identifications of proteins. Proteome analyses can be broadly categorized into
three types of studies: quantitative protein profile comparisons, analysis of
protein-protein interactions, and compositional analysis of simple proteomes or
subproteomes such as organelles or large protein complexes. The complexity of
different types of proteomes, the merits of targeted versus global proteome
studies, and the advantages of alternative separation and analysis technologies
are discussed.

Organelle proteomics.
(Curr Protoc Protein Sci. 2006 Jun;Chapter 24:Unit 24.2.)
Lescuyer P, Chevallet M, Luche S, Rabilloud T.

CEA Grenoble, Grenoble, France.

This unit describes strategies for studying the proteomes of organelles, which is
one example of targeted proteomics. It relies heavily on previously published
units dealing with organelle preparation, protein solubilization, and proteomics
techniques. A specific commentary for organelle proteomics is provided. Specific
protocols for the isolation of nuclei from various sources (cell cultures,
tissues) are also provided.

(Nature reviews on proteomics)
1: Boguski MS, McIntosh MW.
Biomedical informatics for proteomics.
Nature. 2003 Mar 13;422(6928):233-7. Review.

2: Hanash S.
Disease proteomics.
Nature. 2003 Mar 13;422(6928):226-32. Review.

3: Sali A, Glaeser R, Earnest T, Baumeister W.
From words to literature in structural proteomics.
Nature. 2003 Mar 13;422(6928):216-25. Review.

4: Aebersold R, Mann M.
Mass spectrometry-based proteomics.
Nature. 2003 Mar 13;422(6928):198-207. Review.

5: Pandey A, Mann M.
Proteomics to study genes and genomes.
Nature. 2000 Jun 15;405(6788):837-46. Review.

Proteomics books
(Biowww bookshelf)
Books on proteomics research

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