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Dut/ung in vitro Site-directed Mutagenesis Protocol new protocol
Protocol from Bjorkerman lab at CIT. Originally written by M. Davis and P. Bjorkman. ...

Some commercial kits and their brief introduction (biocompare) new protocol
Several commercially available site-directed mutagenesis kits and their brief introduction re given in biocompare.com web site. ...

Site-directed Mutagenesis using PCR new protocol
From: Molecular Biology: Current Innovations and Future Trends. Eds. A.M. Griffin and H.G.Griffin. ISBN 1-898486-01-8 1995 ...

QuikChange(tm) Site-Directed Mutagenesis Kit new protocol
Stratagene's QuickChange kit manual. ...

DpnI mediated site-directed Mutagenesis new protocol
Protocol from Nonet lab at Washington University at St. Louise. A highly effective simple method for making site directed lesions in plasmids without subcloning based on the work of Fisher and Pei (1997). ...

General protocol on construction of mutants, chimeras and truncated recombinant plasmid new protocol
A general overview with some technical details on construction of mutant, chimeras and truncated version of plasmid. (from Liz Greene and Steve Henikoff, Kenesin web site) ...

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening new review
This original article is accessible free from NAR. Nucleic Acids Research, 2003, Vol. 31, No. 3 e11 TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening Lei Young* and Qihan Dong Department of Medic ...

Site-directed mutagenesis troubleshooting
I have a cloned gene. I want to mutate a single base-pair. Can anyone recommend any good protocol. Which kit do you suggest? Malay ...

Internal deletions by PCR troubleshooting
I wonder if anybody could tip me on a good and fast way of making internal deletions in a plasmid by PCR. I have previously done it by introducing new restriction sites by site directed mutagenesis (and then digesting and re-ligating) which takes quite a long ...

Site directed mutagenesis troubleshooting
help for site directed mutagenesis. help for site directed mugagenesis ...

Mutagenesis for deletions troubleshooting
Mutagenesis for deletions w/o kit. Source of method ...

More site-directed mutagenesis protocols

Synonymous (silent), nonsense, missense mutations
(Human Molecular Genetics 2)
9. Instability of the human genome: mutation and DNA repair 9.2. Simple mutations


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