Cell Biology / Cell motility protocols and methods

Technique / Cell Biology / Cell motility

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC protocol
A general protocol from Mitchinson lab at Harvard medical school. Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/depolymerizing factors on microtubules. ...

Standard Fluorescence Motility Assay protocol
Microtubule standard lfurescence motility assay contributed by William Hancock, J. Howard Laboratory (from Kinesin resource page). ...

Microtubule Binding Assay: binding assay protocol
Modified from Huang & Hackney (JBC 269, 16493-501 1994) (Protocol from Kinesin resource page). ...

Microtubule Assembly protocol
A short protocol on microtubule assembly. Protocol Modified from T. Salmon laboratory (from Kinesin resource page). ...

Tubulin preparation from pig brain protocol
Tubulin is labile and yields will be greatly improved by working rapidly in the initial steps of the preparation. Modified from Williams, R.C. Jr. & Lee, J.C. (1982) Meth. Enzymol. 85, 376-385. (from Kinesin resource site). ...

cytoskeleton troubleshooting
I´m seeking for information about the rolr of the cytosceleton in intracellular signal transduction. please send me some information ... ... Click following links for full story. ...

Bionews jobs and tools

Troubleshooting search

Bioscience biotech jobs

Buffer reagents

New research papers

Suggest a new resource

Last update:

08-May-2008 11:32 pm

Related new papers and reviews

Mathematics of cell motility: have we got its number?

Mathematical and computational modeling is rapidly becoming an essential research technique complementing traditional experimental biological methods. However, lack of standard modeling methods, difficulties of translating biological phenomena into mathematical language, and differences in biological and mathematical mentalities continue to hinder the scientific progress. Here we focus on one area-cell motility-characterized by an unusually high modeling activity, largely due to a vast amount of quantitative, biophysical data, 'modular' character of motility, and pioneering vision of the area's experimental leaders. In this review, after brief introduction to biology of cell movements, we discuss quantitative models of actin dynamics, protrusion, adhesion, contraction, and cell shape and movement that made an impact on the process of biological discovery. We also comment on modeling approaches and open questions.