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Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC protocol
A general protocol from Mitchinson lab at Harvard medical school. Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/depolymerizing factors on microtubules. ...

Standard Fluorescence Motility Assay protocol
Microtubule standard lfurescence motility assay contributed by William Hancock, J. Howard Laboratory (from Kinesin resource page). ...

Microtubule Binding Assay: binding assay protocol
Modified from Huang & Hackney (JBC 269, 16493-501 1994) (Protocol from Kinesin resource page). ...

Microtubule Assembly protocol
A short protocol on microtubule assembly. Protocol Modified from T. Salmon laboratory (from Kinesin resource page). ...

Tubulin preparation from pig brain protocol
Tubulin is labile and yields will be greatly improved by working rapidly in the initial steps of the preparation. Modified from Williams, R.C. Jr. & Lee, J.C. (1982) Meth. Enzymol. 85, 376-385. (from Kinesin resource site). ...

cytoskeleton troubleshooting
I´m seeking for information about the rolr of the cytosceleton in intracellular signal transduction. please send me some information ... ... Click following links for full story. ...

More cell motility protocols


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