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James Hardwick's angiotensin assay protocol protocol
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use fewer or more cells, depending on your needs. (Sefton Lab, Salk institute). ...

Steady State ATPase Assays Coupled Enzyme System protocol
The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion of pyruvate to lactate by lactate dehydrogenase (LDH). The latter step requires NADH which is oxidized to NAD+ ...

ATPase assays using 32P-ATP protocol
Two different types of assays are used to determine myosin's ATPase activity. First, the ATPase activity of myosin in high salt is measured in the presence of EDTA, calcium, or magnesium. Myosin's ATPase is highest in EDTA, and lowest in magnesium. Second, the ...

ATPase Assay protocol
The protocol describes how to assay the catalytic activity of purified and reconstituted CFTR. By Ilana Kogan, Mohabir Ramjeesingh and Christine E. Bear, University of Toronto. ...

Yeast Mot1 ATPase assay protocol
PDF protocol from Auble Lab, University of Virginia. ...

Caspase-3 Fluorometric Assay new protocol
This protocol describes detailed procedures on determination of the increased enzymatic activity of the caspase-3 class of proteases in apoptotic cells by fluorometric reaction. (R&D systems) ...

How can I assay translational rate ?? troubleshooting
Some annoying probrems !!Please give me advices eg. drug A induced increased level of TGF-beta protein but do not induced increased expression of TGF-beta mRNA How can I approach the translational control ?? It Seems not by nuclear r ...

Temperature control in the T7 RNA polymerase/promoter system. (For protein expre troubleshooting
Why is the temperature control for growing E. coli under the T7 RNA polymerase/promoter system required? Page 16-6 in "Short Protocols In Molecular Biology 3rd ed. edited by Fred Ausubel et al." explains the procedures for growing E. coli under the T7 ...

More enzyme protocols


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