Technique / Cell Biology / Cell cycle analysis
| The cell cycle consists of four distinct phases: G1 phase, S phase, G2 phase (collectively known as interphase) and M phase. |
Flow Cytometric Protocols for Cell Cycle Analysis
hot
protocol
Protocol from Flow Cytometry Core Facility at PrincetonUniversity.
PROPIDIUM IODIDE STAINING METHOD
ACRIDINE ORANGE STAINING METHOD
HOECHST/DAPI STAINING METHOD
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Cell synchronization protocol
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protocol
Detailed PDF protocol link to LabVelocity.com. Source from BioSource International.
In order to study many of the control mechanisms involved in cell cycle regulation,
it is critical to synchronize the cells so that the distinct phases of the cycle are bei ...
BrdU and PI staining for cell cycle analysis
new
protocol
Fero lab at Fred Hutchinson cancer center. ...
G1/S Phase Block for Cell Synchronization
protocol
This is a protocol using aphidicolin to synchronize whole culture cells in G1/S phase. The amount of aphidicolin (and the time of exposure) may vary depending on the cell line you are working with. (Andrew Murray, Harvard University) ...
Metaphase Block for Cell Synchronization
protocol
A protocol to synchronize cells in G2/M using nocodazole. Protocol from Science Advisory Board. ...
G1/S cell synchnronization using double thymidine block
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protocol
This protocol is designed to synchronize HeLa cells at the G1/S border (actually all G1). It is highly effect and gives almost complete synchronization in the culture. Note that this protocol will not work on cell lines with intact p53 apoptotic responses. (Sc ...
Cell cycle analysis and DNA content protocol
new recommended
review
Review of cell cycle analysis and protocol for DNA content analysis by flow cytometry with detailed FACS analysis illustrations. (Dr.McLeod, Aldaz at UTMD Ander Cancer Center). ...
Reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest points
review
A review by Dr.Stephen Coopter on reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest points. Their application on cell cycle analysis. (PDF file) ...
Cell cycle methods (method booklet)
review
Method booklet on cell cycle analysis (BioSource international).
Content:
Introduction ................................................................................2
I. Cell Proliferation ....................................................... ...
Best way of cell cycle separation
troubleshooting
What's the best way to separate cells based on cell cycle phase?
I read about something called "counterflow centrifugal elutriation", but have
no idea what it is. Can you actually separate cells based on cell cycle phase
using FACS or does it just det ...
Cell-cycle independent internal control genes
troubleshooting
I'm investigating cell cycle progression by measuring changes in the
amount of cyclin mRNA by RT-PCR. For this I need an internal control gene
which is cell-cycle independent, since this is not so for commonly-used
internal references such GAPDH or b- ...
(Molecular Biology of the Cell)
IV. Internal Organization of the Cell 17. The Cell Cycle and Programmed Cell Death
Last update: 
11-Mar-2010 01:15 pm
Cell-cycle inhibitor profiling by high-content analysis.
Adv Exp Med Biol. 2007;604:137-48 Authors: Gasparri F, Ciavolella A, Galvani AAnalysis of cell cycle phases and progression in cultured mammalian cells.
Methods. 2007 Feb;41(2):143-50 Authors: Schorl C, Sedivy JMManipulating the human embryo: cell cycle checkpoint controls.
Cloning. 2000;2(1):1-7 Authors: Fulka J, Tesarík J, Loi P, Moor RMHitting their targets: an emerging picture of E2F and cell cycle control.
Curr Opin Genet Dev. 2004 Oct;14(5):527-32 Authors: Blais A, Dynlacht BDCell cycle-associated proteins in melanotic neuroectodermal tumor of infancy.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999 Oct;88(4):466-8 Authors: de Souza PE, Merly F, Maia DM, Castro WH, Gomez RS