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Metabolic Labeling of Cells with 35S
new recommended
protocol
A protocol from Bjorkman group at the CIT. (pdf format) ...
Metabolic labeling of cell proteins with [35S]-met
new
protocol
Protocol from Hypertension Research Laboratory, UCSD. The original protocol from An Zhou at Johns Hopkins, 1995 ...
Metabolic labeling & Immunoprecipitation
protocol
A metabolic labeling and IP protocol by Jun Takagi from Springer lab at CBR Institute for Biomedical Research.
Cells incorporate 35S-methionine or cysteine during the protein synthesis. Thus it is essential to use Met,Cys-free medium and dialyzed FCS during ...
A method for efficient isotopic labeling of recombinant proteins
protocol
An open access article on A method for efficient isotopic labeling of recombinant proteins.
Reference:
Journal of Biomolecular NMR, 20: 71¨C75, 2001.
Jonathan Marley, Min Lu and Clay Bracken
A rapid and efficient approach for preparing isoto ...
Protein metabolic labeling
troubleshooting
Original review on protein metabolic labelling for proteomics study.
By Robert J. Beynon{ddagger} and Julie M. Pratt, Molecular & Cellular Proteomics 4:857-872, 2005
There is a gradual emergence of the realisation of the advantages of stable isotope labe ...
How to show that the proteins are really labeled?
troubleshooting
We have fed a radiolabeled polymer to some bacteria and then did an
extraction/fractionation on the culture. One of the fractions is a phenol
solution that supposedly contains proteins.
What we want to know is whether the bacteria incorporated a ...
S-35 and autoradiography
troubleshooting
I am looking for experienced opinion about exposure times for S-35
protein labelling followed by IP and exposing to X-ray film versus Phosphorimager. Which of the ... ...
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More protein metabolic labeling protocols
Last update: 
19-May-2013 11:34 pm
