Cell Biology / Metabolic labeling proteins protocols and methods

Technique / Cell Biology / Metabolic labeling proteins

Metabolic Labeling of Cells with 35S new recommended protocol
A protocol from Bjorkman group at the CIT. (pdf format) ...

Metabolic labeling of cell proteins with [35S]-met new protocol
Protocol from Hypertension Research Laboratory, UCSD. The original protocol from An Zhou at Johns Hopkins, 1995 ...

Metabolic labeling & Immunoprecipitation protocol
A metabolic labeling and IP protocol by Jun Takagi from Springer lab at CBR Institute for Biomedical Research. Cells incorporate 35S-methionine or cysteine during the protein synthesis. Thus it is essential to use Met,Cys-free medium and dialyzed FCS during ...

A method for efficient isotopic labeling of recombinant proteins protocol
An open access article on A method for efficient isotopic labeling of recombinant proteins. Reference: Journal of Biomolecular NMR, 20: 71¨C75, 2001. Jonathan Marley, Min Lu and Clay Bracken A rapid and efficient approach for preparing isoto ...

Protein metabolic labeling troubleshooting
Original review on protein metabolic labelling for proteomics study. By Robert J. Beynon{ddagger} and Julie M. Pratt, Molecular & Cellular Proteomics 4:857-872, 2005 There is a gradual emergence of the realisation of the advantages of stable isotope labe ...

How to show that the proteins are really labeled? troubleshooting
We have fed a radiolabeled polymer to some bacteria and then did an extraction/fractionation on the culture. One of the fractions is a phenol solution that supposedly contains proteins. What we want to know is whether the bacteria incorporated a ...

S-35 and autoradiography troubleshooting
I am looking for experienced opinion about exposure times for S-35 protein labelling followed by IP and exposing to X-ray film versus Phosphorimager. Which of the ... ... ...

S-35 pulse labeled membrane protein runs slower?
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in vivo pulse labeled with S-35

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11-May-2008 11:57 am

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Metabolic labeling with amino acids.

Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This unit focuses on pulse-labeling and pulse-chase experiments done with [(35)S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.