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Protocols For Mitochondrial Research
new recommended
protocol
Technical protocols related to mitochondrial protein purification, staining and electrophoresis separation. All protocols are in pdf format. (MitoScience LLC).
Content:
Immunocapture / Immunoprecipitation Protocols
Immunocytochemistry Pr ...
Blue Native Gel Electrophoresis for Thylakoid Protein Complexes
protocol
Protocol from Dr.Kenneth Cline's lab. The original protocol is used for chloroplasts, chromoplasts, and etioplasts study. ...
Blue native polyacrylamide gel electrophoresis: a powerful tool in diagnosis of oxidative phosphoryl
new
protocol
Blue native PAGE (BN-PAGE) gel electrophoresis
Science STKE protocol:
Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for the Identification and Analysis of Multipro ...
Subcellular Fractionation Protocol
hot
protocol
A Short Subcellular Fractionation Protocol. (Finkbeiner lab, UCSF) ...
Mitochondria isolation from tissue or cell protocol
protocol
A detailed protocol on mitochondria isolation from tissue or cell (Mitosciences Inc.)
The key steps when isolating mitochondria from any tissue or cell are always the same: (i) rupturing of cells by mechanical and/or chemical
means and (ii) differential ce ...
Crude Nuclear Extracts
new
protocol
Procedure of nuclear extraction from tissue and monolayer cell culture. (Laboratory of Gene Therapy Research, Copenhagen University Hospital) ...
Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology, Vol 197)
review
Mitochondrial DNA: Metho ...
Cell fractionation based on density gradient
review
A general notes on techniques of cell fractionation. In the study of cell organelles, different fractions of the subcellular particles are routinely separated with a centrifuge. It was by this type of isolation technique that mitochondria were discovered to be ...
Mitochondria: Architecture dictates function
site
Web page containing mitochondria online review. From University of Texas Medical Branch. ...
Enucleation and Fusion Protocol for the Transfer of Mitochondrial DNA to rho-zero Cells
new
site
Enucleation and Fusion Protocol for the Transfer of Mitochondrial DNA to rho-zero Cells
Once week prior to fusion start Rhodamine-6-G treatment of muscle. At the same time plate 2x10^5 of donor cells (5 plates).
1. The day before the actual fusion prepar ...
Golgi apparatus isolation protocol
troubleshooting
An classical method for purification of golgi apparatus enriched fraction from leukaemic cells. (A Warley and G M Cook)
Isolation of a Golgi-apparatus-enriched fraction from leukaemic cells A Warley and G M Cook
Biochem J. 1976 May 15; 156(2): 245–2 ...
Mitochondrion and chloroplast databases
database
From Amo's WWW links page.
GOBASE -
Organelle Genome Database
MitoDat -
Mendelian Inheritance and the Mit ...
Protein subcellular localization prediction tools
new
software
This page is a summary of protein subcellular localization prediction tools and related papers. It is updatd bimonthly. (maintained by Zhiyong, Larry Hunter lab at the UCHSC Center for Computational Pharmacology) ...
Last update: 
19-May-2013 11:34 pm
