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Immunofluorescence Staining & Flow Cytometry of Cell Surface Antigens protocol
Protocol from eBioscience, Inc. ...

FACScan Startup and Shutdown Procedure protocol
From JCSMR FACS Facility. This procedure is for FACScan machine. ...

General cell staining protocol for flow cytometry protocol
From FACS core facility, University of Connecticut Health Center. ...

Cytometer FACScan/Calibur/LSR/LSR-2 Operation Training Guide protocol
From FACS core facility at University of Connecticut Health Center. ...

Labelling of Nuclei with BrdU and PI for FACS protocol
Protocol of Labelling of Nuclei with BrdU and PI for FACS. (Finkbeiner lab, UCSF). ...

96 well microplate intracellular staining for FACS analysis new protocol
Protocol of microplate intracellular staining for FACS analysis from Dr. Cattoretti lab, Columbia University. ...

Flow cytometry protocols new protocol
Flow cytometry protocols from University of Pennsylvania, school of dental medicine. content: Direct Surface Immunophenotyping PI Staining for Cell Cycle Determination PLUS FITC-Surface Staining Three Apoptosis Detection Techniques Hoechst/7 ...

Cell cycle analysis with PI and BrdU new protocol
Cell Cycle Analysis Using Propidium Iodide & Bromodeoxyuridine by W. Roy Overton, Ph.D. Reagent and detailed procedure included. ...

Commonly used Cytometry Apoptosis Detection Techniques new protocol
Hoechst and 7-AAD H342 is DNA stain that binds preferentially to A-T base-pairs without the necessary of permeabilization step. However, it is necessary to set up incubation conditions for efficient internalization of the dye. Hoechst 33342 (Bisbenzi ...

Common Fluorescent Dyes in Flow Cytometry review
An introduction on commonly used fluorescent dyes in FACS analysis (from ebioscience inc). ...

Compensation in Flow Cytometry Analyses review
This method review is written by Mario Roederer. Compensation is probably the least understood process accompanying flow cytometric analyses. Perhaps this is because it is often described with the linear algebra elements needed for its computation, and many of ...

Purdue University Cytometry Laboratories Web Site (PUCL) recommended site
A popular site for cytometry researchers with many valuable resources on cytometry application. ...

Flow Cytometry on the Web site
This is the flow cytometry WWW links list located at the Salk Institute CCMI, La Jolla, California. ...

Collection of cytometry protocols from Janis V. Giorgi Lab site
List of protocols from the Janis V. Giorgi cytometry Lab: Flow Cytometry Glossary Method for EtOH fixation of cells for long-term storage and DNA staining Minimizing Background Staining Blocking FC-Receptor Binding Monoclonal Antibody Staining Proc ...

FACS Sensitivity troubleshooting
Could anyone tell me the limit of sensitivity of fluorescence activated cell sorting? Is it possible to detect, for example,one labelled cell out of a population of 1 million cells? ... ... Click following links for original posts. ...

Optimizing Sorting Experiments troubleshooting
This page listed several factors that need to be considered in order to prepare cells properly for a successful FACS. (TSRI Flow Cytometry Protocols) ...

Table of Fluorochromes software
This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted) From JCSMR FACS lab. ...

More flow cytometry protocols

Determining cell cycle stages by flow cytometry.
(Curr Protoc Cell Biol. 2001 May;Chapter 8:Unit 8.4.)
Darzynkiewicz Z, Juan G, Bedner E.

New York Medical College, Valhalla, New York, USA.

This unit describes assays used to determine the distribution of a population of
cells to the different stages of the cell cycle as analyzed by flow cytometry.
Staining the DNA with different fluorescent dyes, propidium iodide or DAPI, is
one of the most direct ways of staging the cells based on DNA content. These
staining procedures can be used with fixed cells or detergent-permeabilized
cells. Alternatively, live cells can be stained with the membrane-permeable
stain, Hoechst 33242, and sorted on the basis of DNA content for further culture
and characterization. When DNA content measurements are coupled with
immunostaining for cyclin expression, it is possible to reliably detect even more
stages in progression through the cell cycle.

Flow cytometry of apoptosis.
(Curr Protoc Cell Biol. 2004 Feb;Chapter 18:Unit 18.8.)
Pozarowski P, Grabarek J, Darzynkiewicz Z.

School of Medicine, Lublin, Poland.

Common methods applicable to flow cytometry make it possible to: (1) identify and
quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or
necrosis), and (3) study mechanisms involved in cell death. Gross changes in cell
morphology and chromatin condensation, which occur during apoptosis, can be
detected by analysis with laser light beam scattering. Early events of apoptosis,
dissipation of the mitochondrial transmembrane potential and caspase activation,
can be detected using either fluorochrome reporter groups or appropriate
antibodies. Exposure of phosphatidylserine on the exterior surface of the plasma
membrane can be detected by the binding of fluoresceinated annexin V. Another
apoptotic event, DNA fragmentation based on DNA content of cells with fractional
("sub-G1") or DNA strand-break labeling, TUNEL; or In Situ End Labeling, ISEL;.
Still another hallmark of apoptosis is the activation of tissue transglutaminase
(TGase), the enzyme that crosslinks protein and thereby makes them less
immunogenic. The major advantage of flow cytometry in these applications is that
it provides the possibility of multiparametric measurements of cell attributes.

Flow cytometry technique books
(Biowww bookshelf)
flow cytometry FACS techniques

FACS kills culture cells from bone marrow
(Forum)
Troubleshooting on FACS sorted cell culture


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