Home Technique / Immunology / HIV test techniques

More HIV, AIDS protocols

HIV test
(HIV AIDS lab test)
HIV antibody testing

HIV-1 infection can be tested in lab using HIV blood antibody testing technique. HIV test is a two-tiered testing protocol including enzyme immunoassay test followed by the immunoblot assay (western blotting) to detect HIV-1 and HIV-2 from blood sample. The enzyme immunoassay (EIA) and immunochromatographic card assay is the first step HIV test that are very sensitive but less specific. If the EIA test is positive, the second step HIV test of running a western blot is conducted to reduce the the false positive rate. The two step HIV test is therefore very specific for positive detection of HIV infection.

HIV two tiered test (HUP)

(Western blot detection of HIV infection papers)
1: Jamjoom GA, Maatouk J, Gazal M, Damanhouri L, Awliaa A, Ruwaihi N, Bawazeer
M, Halabi H, Adel AA, Abdulla A.
Follow-up of HIV western blot indeterminate results.
Ann Saudi Med. 1997 Sep;17(5):518-21.

2: Dobec M, Naegeli A, Furrer K, Kaeppeli F.
Is Western blot alone sufficient to confirm a reactive result of a
fourth-generation HIV screening assay?
Swiss Med Wkly. 2006 Oct 14;136(41-42):672-3.

3: Sudha T, Lakshmi V, Teja VD.
Western blot profile in HIV infection.
Indian J Dermatol Venereol Leprol. 2006 Sep-Oct;72(5):357-60.

4: Syed IH, Balakrishnan P, Solomon SS, Murugavel KG, Kumarasamy N, Vidya S,
Martin SP, Thyagarajan SP, Mayer KH, Solomon S.
HIV-1 western blot assay: What determines an indeterminate status?
Indian J Med Sci. 2005 Oct;59(10):443-50.

5: Tebourski F, Slim MA, Elgaaied A.
Diagnosis of HIV-1 infection: importance of taking into account the anti-pol
gene product reactivity in the interpretation of Western blot results.
Immunol Lett. 2004 Jun 15;94(1-2):39-41.

6: Turner VF.
HIV western blot test.
Med J Aust. 1994 Jun 20;160(12):807-8.

7: Papadopulos-Eleopulos E, Turner VF, Papadimitriou JM.
Is a positive western blot proof of HIV infection?
Biotechnology (N Y). 1993 Jun;11(6):696-707. Review.

General HIV and AIDS books
(Biowww bookshelf)
HIV AIDS related public health books

HIV lab protocol books
(Biowww bookshelf)
HIV lab method and protocol books

    Last update: RSS feeds 06-Apr-2015 04:45 am