Home Technique / Immunology / HIV test techniques


The AIDS Clinical Trials Group Immunology Laboratory Manual recommended protocol
Collection of concensus immunology protocols in pdf file from ACTG (AIDS clinical trials group). Advanced Flow method Apoptosis Method CD 38 Quantitation Method Cryopreservation and Thawing of PBMC Method CTL Method Dye Dilution Assay-CFSE HIV Neu ...

Propagation of Primary HIV-1 Isolates protocol new protocol
A detailed protocol downloadable in pdf format. The techniques described in this protocol are used to prepare virus supernatant for primary HIV-1 isolates obtained through the UNAIDS Network for HIV Isolation and Characterization. (NIH AIDS Research and Refere ...

Subtyping HIV-1 by Heteroduplex Mobility Assay protocol
A pdf manual for the "HMA HIV-1 env Subtyping Kit. (NIH AIDS research and Reference Reagent Program) ...

Protocol for the Collection, Processing, and Testing HIV-1 Semen protocol
A detailed concensus protocol on HIV-1 semen collection, processing and testing. (NIH AIDS program) ...

HIV Molecular Immunology Database database
The HIV Molecular Immunology Database is an annotated, searchable collection of HIV-1 cytotoxic and helper T-cell epitopes and antibody binding sites. Compendium editors: Bette Korber, Christian Brander, Barton F. Haynes, Richard Koup, John P. Moore, Bruce ...

More HIV, AIDS protocols

HIV test
(HIV AIDS lab test)
HIV antibody testing

HIV-1 infection can be tested in lab using HIV blood antibody testing technique. HIV test is a two-tiered testing protocol including enzyme immunoassay test followed by the immunoblot assay (western blotting) to detect HIV-1 and HIV-2 from blood sample. The enzyme immunoassay (EIA) and immunochromatographic card assay is the first step HIV test that are very sensitive but less specific. If the EIA test is positive, the second step HIV test of running a western blot is conducted to reduce the the false positive rate. The two step HIV test is therefore very specific for positive detection of HIV infection.

Reference:
HIV two tiered test (HUP)


(Western blot detection of HIV infection papers)
1: Jamjoom GA, Maatouk J, Gazal M, Damanhouri L, Awliaa A, Ruwaihi N, Bawazeer
M, Halabi H, Adel AA, Abdulla A.
Follow-up of HIV western blot indeterminate results.
Ann Saudi Med. 1997 Sep;17(5):518-21.

2: Dobec M, Naegeli A, Furrer K, Kaeppeli F.
Is Western blot alone sufficient to confirm a reactive result of a
fourth-generation HIV screening assay?
Swiss Med Wkly. 2006 Oct 14;136(41-42):672-3.

3: Sudha T, Lakshmi V, Teja VD.
Western blot profile in HIV infection.
Indian J Dermatol Venereol Leprol. 2006 Sep-Oct;72(5):357-60.

4: Syed IH, Balakrishnan P, Solomon SS, Murugavel KG, Kumarasamy N, Vidya S,
Martin SP, Thyagarajan SP, Mayer KH, Solomon S.
HIV-1 western blot assay: What determines an indeterminate status?
Indian J Med Sci. 2005 Oct;59(10):443-50.

5: Tebourski F, Slim MA, Elgaaied A.
Diagnosis of HIV-1 infection: importance of taking into account the anti-pol
gene product reactivity in the interpretation of Western blot results.
Immunol Lett. 2004 Jun 15;94(1-2):39-41.

6: Turner VF.
HIV western blot test.
Med J Aust. 1994 Jun 20;160(12):807-8.

7: Papadopulos-Eleopulos E, Turner VF, Papadimitriou JM.
Is a positive western blot proof of HIV infection?
Biotechnology (N Y). 1993 Jun;11(6):696-707. Review.

General HIV and AIDS books
(Biowww bookshelf)
HIV AIDS related public health books

HIV lab protocol books
(Biowww bookshelf)
HIV lab method and protocol books


    Last update: RSS feeds 21-Aug-2014 07:43 am

HIV,-AIDS