Technique / Cell Biology

Research approaches utilizing cell culture techniques to study cell functions and structure. Related cell biology protocols, troubleshootings, reviews and useful tools are provided in this section.

Apoptosis and proliferation (15)

Cell culture (44)

Cell cycle analysis (11)

Cell motility (6)

Extracellular Matrix, cell adhesion and cytoskeleton (5)

In vitro assay and enzyme assay (7)

Metabolic labeling proteins (7)

Protein trafficking (2)

Signal transduction methods (18)

Subcellular fractionation and organelle isolation (12)

Mouse ES cell culture new recommended protocol
ES cell biology techniques Collections of cell biology techniques for mouse ES cell culture edited by Dr.Gerard Lazo at USDA. Content: · MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE · Routine Culturing of ES Cells · ISOLATION OF PR ...

Some cell biology techniques new protocol
Cell biology techniques written by Gerard R. Lazo from USDA. Contents: MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE Routine Culturing of ES Cells ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS MITOMYCIN C TREATMENT OF PMEFs GAMMA IRRA ...

Cell biology protocols from scienceboard.net new protocol
Protocol collections from Science Advisory Board web site. Content: 9/22/2003 Phenol extraction of DNA samples 9/30/2003 Northern Blots 10/1/2003 Alkaline Lysis Plasmid Miniprep 10/16/2003 Chromosome preparation and Fluorescence in- ...

Meyer-Teruel Collection of Protocols new site
Some molecular biology and cell biology lab protcols written by Meyer-Teruel at Stanford University. Content: Cell culture and Transfection RBL cell culture Protocol for freezing down RBL cells Extracellular Hepes buffer 8-well ...

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Preparation of human epidermal keratinocyte cultures
(Curr Protoc Cell Biol. 2004 Feb;Chapter 2:Unit 2.6)


Yamada SS.

National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.

Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.

Basic techniques in mammalian cell tissue culture
(Curr Protoc Cell Biol. 2007 Sep;Chapter 1:Unit 1.1)
Phelan MC.

Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.

Stem cell research books
(Biowww bookshelf)
stem cell related books