Preparation of human epidermal keratinocyte cultures
(Curr Protoc Cell Biol. 2004 Feb;Chapter 2:Unit 2.6)
National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.
Basic techniques in mammalian cell tissue culture
(Curr Protoc Cell Biol. 2007 Sep;Chapter 1:Unit 1.1)
Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.
Stem cell research books (Biowww bookshelf)
stem cell related books