Technique / Cell Biology / Cell culture / Insect cell culture
Insect cell culture protocols and medium
new
protocol
Drosophila cell culture is important technique for both fundamental cell biology research and for producing recombinant proteins in large quantity. Baculovirus system provides investigators a powerful tool for engineering protein expression in insect cells.
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(Selected papers on insect cell culture)
1: Ikonomou L, Schneider YJ, Agathos SN.
Insect cell culture for industrial production of recombinant proteins.
Appl Microbiol Biotechnol. 2003 Jul;62(1):1-20. Epub 2003 May 6. Review.
2: Maranga L, Coroadinha AS, Carrondo MJ.
Insect cell culture medium supplementation with fetal bovine serum and bovine
serum albumin: effects on baculovirus adsorption and infection kinetics.
Biotechnol Prog. 2002 Jul-Aug;18(4):855-61.
3: Pereira CA, Pouliquen Y, Rodas V, Massotte D, Mortensen C, Sogayar MC,
Menissier-de Murcia J.
Optimized insect cell culture for the production of recombinant heterologous
proteins and baculovirus particles.
Biotechniques. 2001 Dec;31(6):1262, 1264, 1266, 1268.
4: Pfeifer TA.
Expression of heterologous proteins in stable insect cell culture.
Curr Opin Biotechnol. 1998 Oct;9(5):518-21.
5: Cowger NL, O'Connor KC, Bivins JE.
Influence of simulated microgravity on the longevity of insect-cell culture.
Enzyme Microb Technol. 1997 Apr;20(5):326-32.
6: Weiss SA, Godwin GP, Gorfien SF, Whitford WG.
Insect cell culture in serum-free media.
Methods Mol Biol. 1995;39:79-95. Review.
7: Weiss SA, Whitford WG, Gorfien SF, Godwin GP.
Insect cell-culture techniques in serum-containing medium.
Methods Mol Biol. 1995;39:65-78. Review.
Last update: 
07-Mar-2010 03:34 am
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Peer-reviewed papers
In situ immune infrared fluorescent staining for detection and quantitation of bluetongue virus in Culicoides insect cell culture.
J Virol Methods. 2009 Jun;158(1-2):110-3 Authors: Mecham JO, Brown PL, McHolland LE