|Some important assays for measuring T and B lymphocyte functions in vitro are listed here. Those include methods for B cell antibody production assay, lymphokine production assay, T and B cell proliferation assay, fluorescence activated cell sorting based on lymphocyte cell-surface antigens and multiparameter flow cytometric analysis for immunophenotyping, as well as in vivo lymphocyte function assays.|
More lymphocyte protocols
Preparation and culture of human lymphocytes.
(Curr Protoc Cell Biol. 2001 May;Chapter 2:Unit 2.2.)
National Institute of Neurological Disorders and Stroke/NIH, Bethesda, Maryland,
This unit presents protocols for preparation of lymphocytes from peripheral
(whole) blood or leucopherisis samples, first by differential centrifugation in
Ficoll-Hypaque to separate them from erythrocytes and granulocytes. Repeated
centrifugation removes the plasma and platelets. Monocyte/macrophage cells and
dendritic cells can be purified from the T and B cell population by exploiting
their adhesion to serum-coated plates in the presence of recombinant IL-3.
Different subpopulations of lymphocytes can be isolated by specific antibodies
bound to magnetic beads.
Figure. Lymphocyte separation by using antibodies coupled to magnetic beads (Immunobiology)
Appendix I. Immunologists' Toolbox Isolation of lymphocytes
Isolation of lymphocytes (Immunobiology)
Appendix I. Immunologists' Toolbox
Last update: 07-Nov-2014 06:54 pm