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Technique / Molecular Biology / PCR / Touchdown PCR

Touchdown PCR technique was developed by Don et al in 1991. The initial cycles of touchdown PCR has annealing temperature higher than the Tm of primer pair and decrease gradually until it reaches Tm. The touchdown PCR allows early phase accumulation of more specific products which then serve as templates for later phase PCR amplification thus can decrease the overall non-specific amplification.

Original paper: 'Touchdown' PCR to circumvent spurious priming during gene amplification new protocol This is the original paper on touchdown PCR technique by Don et al on Nucleic Acids Res. 1991 July 25; 19(14): 4008. A frequently encountered problem in PCR amplification of target gene sequences, especially from complex genomes, is the appearance of spuri ... References Related Books Discussion Related news Job search

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    Last update: 16-May-2008 02:25 am