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PCR purification and storage
protocol
This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts, gel electrophoresis, purification, and storage of PCR products. This is a pdf protocol developed by Jeremy Hasseman from Institute for Genomic Research. (TIGR) ...
PCR products electrophoresis
troubleshooting
I am trying to clearly separate two DNA bands on a gel. The difference
between them is 27 bp, and the overall size is between 100 - 200 bp. I have
tried 5% acrylamide gels, and get very sharp defined bands, but my negative
control lane always looks lo ...
PCR product purification
troubleshooting
After PCR with primers containing restriction sites added on, can you
immediately digest the PCR fragments prior to purifying by gel
extraction? In other words can you avoid an extre gel purification or
PCR clean up step prior to restriction digest of ...
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17-May-2008 04:48 am