The Bradford Assay
The bradford assay for protein concentration measurement (by Brad Olson, University of Nebraska-Lincoln)
Bradford assay uses the absorbance shift of Coomassie brilliant blue G-250 ...
The BCA assay for protein quantification
Protocols and introduction on BCA assay for protein quantification:
BCA assay introduction and guide (John Markwell, University of Nebraska-Linkoln)
The BCA protein assay measures t ...
More protein assay protocols
Assays for determination of protein concentration.
(Curr Protoc Protein Sci. 2007 May;Chapter 3:Unit 3.4.)
Olson BJ, Markwell J.
Michigan State University, East Lansing, Michigan, USA.
Biochemical analysis of proteins relies on accurate quantitation of protein concentration. This unit describes how to perform commonly used protein assays, e.g., Lowry, Bradford, BCA, and UV spectroscopic protein assays. The primary focus of the unit is assay selection, emphasizing sample and buffer compatibility. Protein assay standard curves and data processing fundamentals are discussed in detail. This unit also details high-throughput adaptations of the commonly used protein assays, and also contains a protocol for BCA assay of total protein in SDS-PAGE sample buffer that is used for equal loading of SDS-PAGE gels, which is reliable, inexpensive, and quick.
Last update: 18-May-2015 10:02 pm