Technique / Molecular Biology / PCR / In situ PCR
In situ PCR: Amplification and Detection in a Cellular Context
new
protocol
A general introduction and protocol on performing in situ PCR. (Ernest F. Retzel, Katherine A. Staskus, Janet E. Embretson and Ashley T. Haase, University of Minnesota)
Table of Contents:
Introduction
Technical Aspects/Philosophy
Primer Design ...
In Situ Polymerase Chain Reaction: Past, Present, Future
review
From Roche's Special PCR brouchure by Dr. med. Paul Komminoth Division of Cell and Molecular Pathology, Department of Pathology, University of Zščrich. ...
The foundation of successful RT in situ PCR
review
RT in situ PCR allows for the routine and rapid detection of low copy viral and human RNAs. Success with RT in situ PCR is best accomplished with formalin fixed, paraffin embedded material, which allows the study of archival material. The key variable for RT i ...
RT in situ PCR detection of MART-1 and TRP-2 mRNA in formalin-fixed, paraffin-embedded tissues of melanoma and nevi.
Mod Pathol. 2008 Mar;21(3):326-33 Authors: Itakura E, Huang RR, Wen DR, Paul E, Wünsch PH, Cochran AJ

