Technique / Molecular Biology / PCR / Degenerate PCR
Degenerate PCR
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The identification of novel members of gene families by PCR using degenerate primers has been considered more of an art than a science, so much so that the methods books I've come across have been too timid to discuss the considerations that go into the design ...
Last update: 
13-Mar-2010 05:41 am
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Peer-reviewed papers
A simple novel method for the preparation of noncovalent homodimeric, biologically active human interleukin 10 in Escherichia coli-enhancing protein expression by degenerate PCR of 5' DNA in the open reading frame.
Protein Expr Purif. 2008 Dec;62(2):199-205 Authors: Klompus S, Solomon G, Gertler A