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MicroRNA and siRNA Cloning Method new recommended protocol
MicroRNA and siRNA Cloning Method (Bartel Lab Protocol, MIT). Procedural Outline: 1. Oligo Design 2. ImpA Synthesis 3. Synthesizing Oligos 4. Oligonucleotide Adenylation 5. Small RNA Purification 6. 3' Adaptor Ligation 7. 5' Adaptor Ligation 8. RT-P ...

MicroRNA (miRNA) Northern Blot new protocol
Small RNA Northern blots are actually quite easy, once you are set up for it. In getting set up for it. (McManus, M.T., Sharp lab, MIT) ...

MicroRNA cloning protocol new protocol
MicroRNA (miRNA) cloning protocol from Dr.Victor Ambros's lab at Dartmouth medical college. Section I RNA ligation of 3` linker. Section II. Gel purification of (RNA-to-3’ linker) ligation product. Section III. RNA ligation of 5’ linker (optional, “Nels ...

Introduction to micro-RNA Why It Got Lost in the Shuffle for so long review
A brief Introduction to micro-RNA from the science-projects.com. ...

MicroRNA nomenclature: A uniform system for microRNA annotation new review
A letter by VICTOR AMBROS et al. published on RNA (2003), 9:277–279. (Victor Ambros lab, Dartmouth college). ABSTRACT MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characterist ...

microRNA resource from Ambion site
It currently features reviews, research papers, news on microRNA. (Ambion) ...

The miRNA Registry Database new recommended database
The miRNA Registry has been established with two broad aims: (Welcome Trus Sanger Institute) to provide miRNA gene hunters with unique names for novel miRNA genes prior to publication of results to provide a searchable database of published miRNAs ...

microRNA targets database database
It include human and Drosophila microRNA targets. (www.microrna.org) ...

TarBase database of experimentally supported animal microRNA targets new database
Reference: RNA. 2005 Dec 22; TarBase: A comprehensive database of experimentally supported animal microRNA targets Sethupathy P, Corda B, Hatzigeorgiou AG. MicroRNAs (miRNAs) are ~22-nt RNA segments that are involved in the regulation of prote ...

microRNA Target Detection new software
miRanda is an algorithm for finding genomic targets for microRNAs. This algorithm has been written in C and is available as an open-source method under the GPL. MiRanda was developed at the Computational Biology Center of Memorial Sloan-Kettering Cancer Center ...

MiRscan: miRNA hairpins scoring tool new software
If you enter the sequences of two hairpins, MiRscan will assign them a score, based on their similarity to 50 pairs of experimentally verified C. elegans/C. briggsae microRNA hairpins. (MIT) References: Lim, L. P.*, Lau, N. C.*, Weinstein, E. G.*, Abdelh ...

MiRAlign: MicroRNA Identification Based on Sequence and Structure Alignment new software
MiRAlign is a microRNA identification tool based on sequence and structure alignments. (Tsinghua University, Beijing, China) Reference: Xiaowo Wang, Jing Zhang, Fei Li, Jin Gu, Tao He, Xuegong Zhang, and Yanda Li. MicroRNA identification based on seque ...

More mirna protocols

Analysis of small endogenous RNAs.
(Curr Protoc Mol Biol. 2008 Jan;Chapter 26:Unit26.7.)
Lau NC.

Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts,
USA.

Eukaryotic cells express small noncoding RNAs to silence target genes or regulate
chromatin domains. MicroRNAs (miRNA) are endogenous small RNAs that are thought
to each regulate multiple mRNA targets. To gain a deeper understanding of
processes regulated by small noncoding RNAs, techniques are required to identify
and detect them. This unit describes standard laboratory methods for and comments
about high-throughput technologies for the identification and detection of small
RNAs. This unit also outlines a cell-based reporter gene assay for assessing the
regulatory potential of a microRNA on a candidate mRNA target.


(miRNA or microRNA technique reviews)
1: Nilsen TW.
Mechanisms of microRNA-mediated gene regulation in animal cells.
Trends Genet. 2007 Mar 15; [Epub ahead of print]

2: Wang Y, Stricker HM, Gou D, Liu L.
MicroRNA: past and present.
Front Biosci. 2007 Jan 1;12:2316-29.

3: Kosik KS.
The neuronal microRNA system.
Nat Rev Neurosci. 2006 Dec;7(12):911-20. Review.

4: Engels BM, Hutvagner G.
Principles and effects of microRNA-mediated post-transcriptional gene
regulation.
Oncogene. 2006 Oct 9;25(46):6163-9. Review.

5: Ying SY, Chang DC, Miller JD, Lin SL.
MicroRNA protocols. Perspectives.
Methods Mol Biol. 2006;342:351-8. Review.

6: Ishizuka A, Saito K, Siomi MC, Siomi H.
In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell
lysates.
Methods Mol Biol. 2006;342:277-86. Review.

7: Chen CH, Guo M, Hay BA.
Identifying microRNA regulators of cell death in Drosophila.
Methods Mol Biol. 2006;342:229-40. Review.

8: Griffiths-Jones S.
miRBase: the microRNA sequence database.
Methods Mol Biol. 2006;342:129-38. Review.

9: Smalheiser NR, Torvik VI.
Complications in mammalian microRNA target prediction.
Methods Mol Biol. 2006;342:115-27. Review.

10: John B, Sander C, Marks DS.
Prediction of human microRNA targets.
Methods Mol Biol. 2006;342:101-13. Review.

11: Zeng Y, Cullen BR.
Recognition and cleavage of primary microRNA transcripts.
Methods Mol Biol. 2006;342:49-56. Review.

12: Gregory RI, Chendrimada TP, Shiekhattar R.
MicroRNA biogenesis: isolation and characterization of the microprocessor
complex.
Methods Mol Biol. 2006;342:33-47. Review.

13: Krol J, Krzyzosiak WJ.
Structure analysis of microRNA precursors.
Methods Mol Biol. 2006;342:19-32. Review.

14: Ying SY, Chang DC, Miller JD, Lin SL.
The microRNA: overview of the RNA gene that modulates gene functions.
Methods Mol Biol. 2006;342:1-18. Review.

15: Chang K, Elledge SJ, Hannon GJ.
Lessons from Nature: microRNA-based shRNA libraries.
Nat Methods. 2006 Sep;3(9):707-14. Review.

16: Zhang B, Pan X, Anderson TA.
MicroRNA: a new player in stem cells.
J Cell Physiol. 2006 Nov;209(2):266-9. Review.

17: le Sage C, Agami R.
Immense promises for tiny molecules: uncovering miRNA functions.
Cell Cycle. 2006 Jul;5(13):1415-21. Epub 2006 Jul 1. Review.

18: Rajewsky N.
microRNA target predictions in animals.
Nat Genet. 2006 Jun;38 Suppl:S8-13. Review.

19: Berezikov E, Cuppen E, Plasterk RH.
Approaches to microRNA discovery.
Nat Genet. 2006 Jun;38 Suppl:S2-7. Review.

20: Kim VN, Nam JW.
Genomics of microRNA.
Trends Genet. 2006 Mar;22(3):165-73. Epub 2006 Jan 30. Review.

21: Kong Y, Han JH.
MicroRNA: biological and computational perspective.
Genomics Proteomics Bioinformatics. 2005 May;3(2):62-72. Review.

22: Chen PY, Meister G.
microRNA-guided posttranscriptional gene regulation.
Biol Chem. 2005 Dec;386(12):1205-18. Review.

23: Pillai RS.
MicroRNA function: multiple mechanisms for a tiny RNA?
RNA. 2005 Dec;11(12):1753-61. Review.

24: Du T, Zamore PD.
microPrimer: the biogenesis and function of microRNA.
Development. 2005 Nov;132(21):4645-52. Review.

25: Vaucheret H.
MicroRNA-dependent trans-acting siRNA production.
Sci STKE. 2005 Sep 6;2005(300):pe43. Review.

26: Wienholds E, Plasterk RH.
MicroRNA function in animal development.
FEBS Lett. 2005 Oct 31;579(26):5911-22. Epub 2005 Aug 10. Review.

27: Tang G.
siRNA and miRNA: an insight into RISCs.
Trends Biochem Sci. 2005 Feb;30(2):106-14. Review.

28: Lai EC.
Predicting and validating microRNA targets.
Genome Biol. 2004;5(9):115. Epub 2004 Aug 31. Review.

29: Nelson P, Kiriakidou M, Sharma A, Maniataki E, Mourelatos Z.
The microRNA world: small is mighty.
Trends Biochem Sci. 2003 Oct;28(10):534-40. Review.

30: Brennecke J, Cohen SM.
Towards a complete description of the microRNA complement of animal genomes.
Genome Biol. 2003;4(9):228. Epub 2003 Aug 21. Review.



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