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More restriction enzyme digestion protocols

Figure. Relative positions of different DNA forms of plasmid on agarose gel
(Donis-keller lab manual)
Uncut plasmid DNA can be in any of five forms - nicked circular linear covalently closed supercoiled or circular single-stranded

Protein precipitation after dialysis
dialysed o/n in dialisys buffer contained 50mM Tris pH 8.0, 50 mM NaCl

Secondary Ab background
Secondary antibody caused high background for western blot

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