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Fundamental Techniques in
Cell Culture - a Laboratory Handbook
recommended
review
Handbook from Sigma on principle and protocols of cell culture.
Content:
1.0 The ECACC and its Collections
2.0 Design and Equipment for the Cell Culture Laboratory
3.0 Safety Aspects of Cell Culture
4.0 Sourcing of Cell Lines
5 ...
Mouse Genetics: Concepts and Applications
recommended
review
A online book originally by Lee M. Silver (Oxford University Press, 1995). Adapted for the Web by: Mouse Genome Informatics, The Jackson Laboratory.
Table of content:
Chapter 1
An Introduction to Mice
1.1 Of mice, men, and a woman
1.1.1 The o ...
pET vector Expression System Manual, 11th Edition
new recommended
review
The pET System Manual is a comprehensive user guide and technical reference for the pET vectors and pET expressions systems. User Protocols contain protocols, storage instructions and additional product information for Novagen products. (Novagen EMD) ...
Cloning Enzymes (Promega tech book)
review
Cloning Enzymes, one in the Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Enzymes that modify nucleic acids provide the foundation for many molecular biology techniques: enzymes that are used to synthesize ...
DNA Analysis Notebook (Promega)
review
DNA analysis is the starting point for many molecular biology procedures. The DNA Analysis Notebook provides an introduction to DNA procedures such as purifying genomic DNA, amplifying via PCR, retrieving DNA from PCR and cloning PCR DNA. In addition, the DNA ...
In Vitro Expression (Promgea)
review
In Vitro Expression tools are used in functional genomics as a bridge between traditional genomics and proteomics. This comprehensive guide details the technologies and applications associated with in vitro expression. Valuable references and schematics are al ...
Nucleic Acid Purification Systems (Promega)
review
The isolation and purification of DNA is crucial to many applications in molecular biology. Over the past decade, the purification of DNA has evolved from a multi-step process involving organic chemicals to a much simpler process, that is safer and faster. Pro ...
PCR Protocols & Reference
(Promega)
review
PCR is central to molecular biology. Although in principle a simple process, PCR can be difficult to optimize. Furthermore, new variations of the technique appear at an alarming rate. Our guide provides a convenient source of information on this technique, inc ...
Restriction Enzymes Resource (Promega)
review
Table of Contents: (Promega)
1. General Information
1.1 History
1.2 Types, Definitions and Genomic Organization
1.3 Restriction Enzyme Structure and Mechanism of Action
1.4 Star Activity
1.5 Site Preferences and Turbo? Restriction Enzymes ...
Signal Transduction Resource (Promega)
review
Promega's Signal Transduction Resource Guide features overviews of key signaling pathways and the cell signaling reagents Promega offers to help unravel these complex pathways. Signaling pathways include the Mitogen Activated Protein Kinase pathways, the Prote ...
Transfection (Promega)
review
Promega's family of transfection reagents are highly efficient, fast, easy-to-use and a tested complement to our reporter systems. Our commitment to your success in transfection and eukaryotic expression studies is reflected in the detail found in this guide, ...
Apoptosis (Promega guide chaptor 3)
review
Promega tech book on apoptosis.
Content:
Introduction
Detecting Caspase Activity and Activation
Detecting Cell Death Using Mitochondrial Markers
Detecting Apoptosis by Measuring Changes in the Cell Membrane
Using DNA Fragmentation to Detect Ap ...
Free Life Science quarterly journal from Sigma
review
You can also register to get reprint copy from Sigma web site.
It features articles from following area:
Automated DNA Sequencing DNA and RNA Purification
Cell Biology Drug Discovery
Cell Culture Gene Expression
Cell Signaling PCR
Cloning and Exp ...
LabFAQ: working with DNA RNA Protein
new
review
A online tech note from Roche on general guidance of DNA, RNA, Protein related works. It is a collection of reference information specially designed to assist you during your day-to-day laboratory work. It can also be downloaded as pdf file (Roche) ...
PCR Application Manual
new
review
A 321 pages of PCR application manual from Roche Applied Biosciences. ...
Western Blotting Handbook and Troubleshooting Guide
new
review
Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Western Blotting Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Transfer Protein to a Membrane . . . . . . ...
RNA analysis notebook
new
review
This is an online version of RNA analysis methods technique book from Promega. You can also download the PDF file.
RNA analysis is the starting point for many molecular biology procedures. The RNA Analysis Notebook provides an introduction to RNA procedures ...
Free magazine: Nature methods (Nature methods)Complimentary subscriptions to Nature Methods are available in North America and Europe and are provided to individuals involved in research within the life sciences or chemistry. Subscriptions are subject to the publisher’s acceptance. Free magazine: BioTechniques (BioTechniques)BioTechniques® has provided the international community of life science researchers free access to peer-reviewed research articles on a monthly basis. Free magazine: BioPharm International (BioPharm International)BioPharm International provides a forum for its readers to exchange practical, hands-on information for developing and manufacturing pharmaceutical products through biotechnology; and serves as a supportive, authoritative voice in the evolving business, regulatory, scientific, and technical areas of the biopharmaceutical industry. Free magazine: Bio-ITworld (Bio-ITworld)The convergence of information technology and the life sciences has created a new professional community and discipline -Bio-IT - with unique information needs
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